甜菜镉胁迫应答基因ZAT10的克隆与功能预测  

Cloning and Functional Prediction of the Sugar Beet Cadmium Stress-responsive Gene ZAT10

作　　者：姚琦 石俊婷 鲁振强[1] 刘大丽[2,3] YAO Qi;SHI Junting;LU Zhenqiang;LIU Dali(School of Life Sciences,Heilongjiang University/Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education/Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region/Key Laboratory of Molecular Biology,College of Heilongjiang Province,Harbin 150080;National Beet Medium-term Gene Bank/Heilongjiang University,Harbin 150080;Key Laboratory of Sugar Beet Genetics and Breeding,Colleges of Heilongjiang Province/College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080)

机构地区：[1]黑龙江大学生命科学学院/农业微生物技术教育部工程研究中心/黑龙江省寒区植物基因与生物发酵重点实验室/黑龙江省普通高校分子生物学重点实验室,哈尔滨150080 [2]国家甜菜种质中期库/黑龙江大学,哈尔滨150080 [3]黑龙江省普通高等学校甜菜遗传育种重点实验室/黑龙江大学现代农业与生态环境学院,哈尔滨150080

出　　处：《中国糖料》2024年第3期1-9,共9页Sugar Crops of China

基　　金：财政部和农业农村部国家现代农业产业技术体系(糖料)建设项目(CARS-17);甜菜种质资源安全保存项目(19230841);甜菜种质资源精准鉴定项目(19240700);黑龙江省高校科研业务费项目(2022-KYYWF-1070);黑龙江省自然科学基金(LH2023C090)资助。

摘　　要：【目的】为了预测甜菜锌指蛋白BvZAT10(LOC 104901462)的结构特点和功能。【方法】以甜菜‘780016B/12优’为试验材料,对BvZAT10基因进行了克隆及生物信息学分析,并利用甜菜响应镉胁迫的转录表达谱数据探究了该基因在镉胁迫下的应答特性。【结果】BvZAT10编码区全长729 bp,编码242个氨基酸,是一种碱性不稳定亲水性蛋白,具有2个C2H2型锌指结构域。该基因编码蛋白共有36处磷酸化修饰位点,蛋白结构以无规则卷曲为主,系统发育分析发现BvZAT10与菠菜和藜麦的ZAT10亲缘关系最近。BvZAT10启动子序列包含植物激素响应、光响应及抗逆相关的多种作用元件。在1、3、5 mmol/L CdCl_(2)处理下,BvZAT10及其预测调控的大部分靶基因,如金属耐受蛋白11(BVRB_2g037080)和ABCG家族成员22(BVRB_5g109050)等,均受到了镉胁迫的正向调控。【结论】推测BvZAT10可能直接或间接地在甜菜应答镉胁迫中发挥重要作用,可将其作为潜在优势抗逆基因深入开展后续研究。【Objective】To predict the structural characteristics and functions of sugar beet zinc finger protein BvZAT10(LOC 104901462),【Methods】using sugar beet‘780016B/12 superior’as experimental material,BvZAT10 gene was cloned and subjected to bioinformatics analysis,and the transcriptional expression profile data of sugar beet in response to cadmium stress were used to explore the gene response characteristics under cadmium stress.【Results】The coding region of BvZAT10 is 729 bp in length,encoding 242 amino acids.It is an alkaline,unstable hydrophilic protein with two C2H2-type zinc finger domains.The protein encoded by this gene has a total of 36 phosphorylation modification sites,and the protein structure is mainly characterized by irregular coiling.Phylogenetic analysis revealed that BvZAT10 is most closely related to ZAT10 in spinach and quinoa.The BvZAT10 promoter sequence contains multiple functional elements related to plant hormone response,light response,and stress resistance.Under 1,3 and 5 mmol/L CdCl_(2) treatments,BvZAT10 and most of its predicted regulatory target genes,such as metal tolerance protein 11(BVRB_2g037080)and ABCG family member 22(BVRB_5g109050),were positively regulated by cadmium stress.【Conclusion】It is speculated that BvZAT10 may play an important role directly or indirectly in sugar beets response to cadmium stress and could be further studied as a potential dominant stress-tolerant gene.

关 键 词：甜菜 镉胁迫 ZAT10 基因克隆 功能预测 

分 类 号：S566.3[农业科学—作物学]