XRN4/EIN5调控拟南芥对盐胁迫和ABA的耐受性  

XRN4/EIN5 Regulates Tolerance to Salt Stress and ABA in Arabidopsis thaliana

作　　者：刘艳[1] 王瑞娟 苏龙 陈浩[1] 翟一凡[1] 郑礼[1] 代晓彦[1] Liu Yan;Wang Ruijuan;Su Long;Chen Hao;Zhai Yifan;Zheng Li;Dai Xiaoyan(Institute of Plant Protection,Shandong Academy of Agricultural Sciences/Key Laboratory of Natural Enemy Insects,Ministry of Agriculture and Rural Affairs,Jinan 250100,China)

机构地区：[1]山东省农业科学院植物保护研究所/农业农村部天敌昆虫重点实验室,山东济南250100

出　　处：《山东农业科学》2024年第6期40-46,共7页Shandong Agricultural Sciences

基　　金：山东省农业科学院农业科技创新工程项目(CXGC2023F04)。

摘　　要：XRN4/EIN5是植物的5′-3′核酸外切酶,主要参与细胞质中RNA的降解,同时也是乙烯信号通路的核心基因,在植物的生长发育和乙烯信号转导中起着重要作用。为探究该基因在植物抗逆性方面的作用,以XRN4/EIN5 T-DNA插入突变体ein5-6为材料,设计不同浓度NaCl(0、50、100、150、200 mmol/L)和ABA(0、0.10、0.25、0.50、1.00、1.50μmol/L)处理,基于拟南芥种子发芽率、子叶绿化率、叶片数、叶片面积进行统计分析,结果发现,当盐浓度低于50 mmol/L时,ein5-6的种子发芽率、子叶绿化率、叶片数和叶片面积比野生型Col-0高;当盐浓度达到100 mmol/L时,ein5-6的种子发芽率和子叶绿化率在发育前期比Col-0高,60 h和96 h之后比Col-0低,而二者的叶片数和叶片面积没有差异;当盐浓度达到150 mmol/L及以上时,ein5-6的种子发芽率、子叶绿化率、叶片数和叶片面积比Col-0低。另外,ein5-6对ABA与盐胁迫响应的趋势一致,ABA浓度较低时ein5-6的子叶绿化率高于Col-0,但随着处理时间的延长和ABA浓度的增加,其子叶绿化率越来越低,最终明显低于Col-0。表明与野生型相比,ein5-6对盐胁迫和ABA处理更敏感,推测XRN4/EIN5参与了盐胁迫和ABA响应的调节。XRN4/EIN5 is a 5′⁃3′exonuclease in plants,which is mainly involved in degradation of RNA in cytoplasm,and also is a core gene of ethylene signaling pathway.Therefore,it plays important roles in plant growth and development and ethylene signaling transduction.In order to explore its function on stress resist⁃ance,the XRN4/EIN5 T⁃DNA insertion mutant ein5⁃6 was used as material,and different concentrations of NaCl(0,50,100,150,200 mmol/L)and ABA(0,0.10,0.25,0.50,1.00,1.50μmol/L)were set for the treatments.Based on the results of seed germination rate,cotyledon greening rate,leaf number and leaf ar⁃ea,it was found that when the salt concentration was lower than 50 mmol/L,the germination rate,cotyledon greening rate,leaf number and leaf area of ein5⁃6 were higher than those of the wild⁃type Col⁃0;when the salt concentration reached 100 mmol/L,the germination rate and cotyledon greening rate of ein5⁃6 were higher at early stage of growth but lower after treated for 60 h and 96 h compared with Col⁃0,and there was no differ⁃ence in leaf number and leaf area between them.When the salt concentration reached 150 mmol/L and above,the germination rate,cotyledon greening rate,leaf number and leaf area of ein5⁃6 were lower than those of Col⁃0.In addition,the response of ein5⁃6 to ABA was consistent with that to salt stress.When ABA concentra⁃tion was low,the cotyledon greening rate of ein5⁃6 was higher than that of Col⁃0,but that decreased with the extension of treating time and the increase of ABA concentration,and finally obviously lower than that of Col⁃0.These results indicated that ein5⁃6 was more sensitive to the salt stress and ABA treatment,so it was speculated that XRN4/EIN5 be involved in the regulation of salt stress and ABA stress responses.

关 键 词：XRN4/EIN5 盐胁迫 ABA 拟南芥 耐受性 

分 类 号：S567.239[农业科学—中草药栽培] Q943.2[农业科学—作物学]