淫羊藿苷调节SDF-1/CXCR4信号通路对多囊卵巢综合征大鼠卵巢颗粒细胞凋亡的影响  

The effect of icariin on apoptosis of ovarian granulosa cells in polycystic ovary syndrome rats by regulating the SDF-1/CXCR4 signaling pathway

作　　者：徐琼芳 钟斐[2] 李子帅 XU Qiongfang;ZHONG Fei;LI Zishuai(Medical College of Yongzhou Vocational Technical College,Yongzhou 425100,China;Department of Obstetrics and Gynecology,Quanzhou Hospital of Traditional Chinese Medicine)

机构地区：[1]永州职业技术学院医学院,425100 [2]泉州市中医院中医妇产科

出　　处：《天津医药》2024年第7期727-732,共6页Tianjin Medical Journal

基　　金：湖南省教育厅科学研究项目(21C1525)。

摘　　要：目的探讨淫羊藿苷调节基质细胞衍生因子-1(SDF-1)/CXC型趋化因子受体4(CXCR4)信号轴对多囊卵巢综合征(PCOS)大鼠卵巢颗粒细胞凋亡的影响。方法将从PCOS模型组大鼠卵巢组织中成功分离的颗粒细胞分为PCOS组、淫羊藿苷低剂量组、淫羊藿苷中剂量组、淫羊藿苷高剂量组、重组大鼠SDF-1蛋白(Rr-SDF-1)组、淫羊藿苷高剂量+Rr-SDF-1组,另取从正常对照组大鼠卵巢组织中成功分离的颗粒细胞作为对照组。酶联免疫吸附试验(ELISA)检测卵巢颗粒细胞上清液中卵泡刺激素(FSH)、睾酮(T)、黄体生成素(LH)水平;CCK-8法、EdU染色检测颗粒细胞增殖;流式细胞术检测颗粒细胞凋亡;Western blot检测颗粒细胞中Bcl-2相关X蛋白(Bax)、裂解的胱天蛋白酶-3(Cleaved Caspase-3)、SDF-1、CXCR4蛋白表达。结果与对照组比较,PCOS组FSH水平、光密度(OD)_(450)值、EdU阳性细胞率降低,T、LH水平、细胞凋亡率、Bax、Cleaved Caspase-3、SDF-1、CXCR4蛋白表达水平升高(P<0.05)。与PCOS组比较,淫羊藿苷低、中、高剂量组FSH水平、OD_(450)值、EdU阳性细胞率均依次升高,T、LH水平、细胞凋亡率、Bax、Cleaved Caspase-3、SDF-1、CXCR4蛋白表达水平均依次降低;Rr-SDF-1组对应指标变化趋势与上述相反(P<0.05)。与淫羊藿苷高剂量组比较,淫羊藿苷高剂量+Rr-SDF-1组FSH水平、OD_(450)值、EdU阳性细胞率降低,T、LH水平、细胞凋亡率、Bax、Cleaved Caspase-3、SDF-1、CXCR4蛋白表达水平升高(P<0.05)。结论淫羊藿苷可能通过抑制SDF-1/CXCR4信号通路抑制PCOS大鼠卵巢颗粒细胞凋亡。Objective To investigate the effect of icariin on apoptosis of ovarian granulosa cells in rats with polycystic ovarian syndrome(PCOS)by regulating stromal cell-derived factor-1(SDF-1)/CXC chemokine receptor-4(CXCR4)signal axis.Methods Granular cells successfully isolated from ovarian tissue of rats in the PCOS model group were grouped into the PCOS group,the low-dose icariin group,the medium-dose icariin group,the high-dose icariin group,the Rr-SDF-1 group and the high-dose icariin+Rr-SDF-1 group.Granular cells successfully isolated from ovarian tissue of rats in the normal control group were taken as the control group.Enzyme-linked immunosorbent assay(ELISA)was applied to detect levels of follicle-stimulating hormone(FSH),testosterone(T)and luteinizing hormone(LH)in supernatant of ovarian granulosa cells.CCK-8 method and EdU staining were applied to detect granulosa cell proliferation.Flow cytometry was applied to detect granulosa cell apoptosis.Western blot assay was applied to detect the expression of Bcl-2 associated X(Bax),Cleaved aspartate-specific cysteine protease-3(Cleaved Caspase-3),SDF-1,and CXCR4 proteins in granulosa cells.Results Compared with the control group,the FSH level,OD_(450) value and EdU positive cell rate were decreased in the PCOS group,and T and LH levels,apoptosis rate,Bax,Cleaved Caspase-3,SDF-1 and CXCR4 protein expression increased(P<0.05).Compared with PCOS group,the FSH level,OD_(450) value and EdU positive cell rate were increased sequentially in the icariin low,medium and high dose groups,T and LH levels,apoptosis rate,Bax,Cleaved Caspase-3,SDF-1 and CXCR4 protein expression levels were decreased sequentially,and change trends of corresponding indicators were opposite to the above in the Rr-SDF-1 group(P<0.05).Compared with the high-dose icariin group,FSH level,OD_(450) value and EdU positive cell rate were decreased in the high-dose icariin+Rr-SDF-1 group,and T and LH levels,apoptosis rate,Bax,Cleaved Caspase-3,SDF-1 and CXCR4 protein expression levels were increased(P<0.05).Co

关 键 词：淫羊藿苷 基质细胞衍生因子-1/CXC型趋化因子受体4信号通路 多囊卵巢综合征 颗粒细胞 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]