黄色小长蝽的线粒体基因组测序和分析  

The Mitochondrial Genome Sequencing and Analysis of Nysius inconspicuus

作　　者：林兴雨 席玉强[1] 宋南[1] 尹新明[1] LIN Xingyu;XI Yuqiang;SONG Nan;YIN Xinming(Henan International Laboratory for Green Pest Control/Henan Engineering Laboratory of Pest Biological Control/College of Plant Protection,Henan Agricultural University,Zhengzhou 450046,China)

机构地区：[1]河南绿色害虫防治国际实验室/河南害虫生物防治工程实验室/河南农业大学植物保护学院,河南郑州450046

出　　处：《河南农业科学》2024年第6期91-99,共9页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(U1904104);国家现代农业体系研究项目(CARS-27)。

摘　　要：为探究黄色小长蝽(Nysius inconspicuus)的线粒体基因组结构及长蝽科(Lygaeidae)昆虫的系统发育关系,利用二代测序方法获得黄色小长蝽线粒体全基因组序列,在系统发育分析中使用长蝽科14个物种作为内群,并选择大眼长蝽科(Geocoridae)的1个物种作为外群,利用最大似然法和贝叶斯法重建长蝽科的系统发育关系。结果表明,黄色小长蝽的线粒体基因组包含37个基因(13个蛋白质编码基因、22个转运RNA基因和2个核糖体RNA基因)和1个非编码控制区,全长为14 893 bp(GenBank登录号:OR099699)。黄色小长蝽线粒体基因组的13个蛋白质编码基因除cox1和nad6利用TTG、nad4l利用AAC作为起始密码子,其余蛋白质编码基因都是以ATN开头,终止密码子除cox1、cox2、nad3和nad1以不完整的T作为结尾,其余蛋白质编码基因都是以TAA结尾。此外,基于3种不同的数据矩阵,分别采用2种系统发育分析方法得到的系统发育树结果基本一致。To explore the mitochondrial genome structure of Nysius inconspicuus and the phylogeny of Lygaeidae,we newly sequenced the complete mitochondrial genome of N.inconspicuus using next‑generation sequencing methods.In phylogenetic analyses,we selected 14 representative species of Lygaeidae as ingroups.One species of Geocoridae was used as outgroup.We reconstructed the phylogenetic relationships using Maximum likelihood and Bayesian inference methods.The results indicated that the mitochondrial genome of N.inconspicuus encompassed 37 genes(13 protein‑coding genes,22 transfer RNA genes,and 2 ribosomal RNA genes)along with 1 non‑coding control region,spanning a total length of 14893 bp(GenBank accession number:OR099699).In the mitochondrial genome of N.inconspicuus,the start codons of protein‑coding genes were TTG for cox1 and nad6,AAC for nad4l,while the remaining protein‑coding genes began with ATN.As for the stop codons,cox1,cox2,nad3,and nad1 ended with an incomplete T,whereas the rest of protein‑coding genes terminated with TAA.In addition,based on three different data matrices,we employed two phylogenetic analysis methods,resulting in largely consistent phylogenetic trees.

关 键 词：黄色小长蝽 半翅目 长蝽科 线粒体基因组 系统发育 

分 类 号：S433[农业科学—农业昆虫与害虫防治] Q969[农业科学—植物保护]