色季拉山杜鹃花属植物叶片解剖结构特征与抗逆性评价  

Anatomical Structure and Stress Resistance Evaluation of Leaves of Rhododendron L.in the Shergyla Mountain

作　　者：郭文文 卓么草 韩嘉华 方江平[2] GUO Wenwen;ZHUO Mecao;HAN Jiahua;FANG Jiangping(Research Institute of Tibet Plateau Ecology,Tibet Agriculture and Animal Husbandry University(National Forest Ecosystem Observation&Research Station of Nyingchi Tibet/Key Laboratory of Forest Ecology in Tibet Plateau,Ministry of Education),Nyingchi 860000,China;School of Ecology and Environment,Tibet University,Lhasa 850000,China;Pai Town Agricultural and Animal Husbandry Comprehensive Service Center,Milin 860500,China)

机构地区：[1]西藏农牧学院高原生态研究所(西藏林芝高山森林生态系统国家野外科学观测研究站/西藏高原森林生态教育部重点实验室),西藏林芝860000 [2]西藏大学生态环境学院,西藏拉萨850000 [3]派镇农牧综合服务中心,西藏米林860500

出　　处：《河南农业科学》2024年第6期111-119,共9页Journal of Henan Agricultural Sciences

基　　金：西藏林业经济系统生态补偿研究项目(605516001);拉萨南北山造林主要乡土树种培育示范中心建设项目(藏财科教指2023-17号)。

摘　　要：研究杜鹃花属植物叶片解剖结构的种间差异及其环境响应策略,能够为该属植物的适应性进化研究提供基础资料。以色季拉山5种杜鹃花属植物叶片为研究对象,采用石蜡切片技术,对物种间各解剖结构的差异性和抗逆性进行研究。结果表明,5种杜鹃花属植物叶片均为典型的异面叶,由叶脉、表皮和叶肉三部分组成;叶片厚度(LLT)、上角质层厚度(LUCT)、上表皮厚度(LUET)、栅栏组织厚度(LPMT)、海绵组织厚度(LSMT)与组织结构紧密度(LPMT/LT)种间差异显著(P<0.05);主成分分析结果显示,下表皮厚度(LLET)、LSMT、LPMT/LT、栅海比(LPMT/SMT)和主脉突起度(LMPD)可作为5种杜鹃花属植物叶片解剖结构抗逆性评价的关键指标;5种杜鹃花属植物的抗逆性排序依次为:雪层杜鹃(RNH)>黄杯杜鹃(RWW)>散鳞杜鹃(RBH)>林芝杜鹃(RNR)>雪山杜鹃(RAB)。综上所述,RNH表现出较强的抗逆性,可在杜鹃花品种选育方面加以利用。Research on the variations in anatomical structure among different species of Rhododendron L.and environmental response strategies can offer fundamental data for the investigation of plant adaptive evolution.The paraffin sealing technique was used to examine the difference and stress resistance of each anatomical structure in five different species of Rhododendron L.found in the Shergyla Mountain.The results showed that,five Rhododendron L.species exhibited typical ectopic leaves,made up of three parts:mesophyll,epidermis and veins.Significant differences were observed among the species in terms of leaf thickness(LLT),upper cuticle thickness(LUCT),upper epidermis thickness(LUET),palisade tissue thickness(LPMT),spongy tissue thickness(LSMT),and tightness of leaf palisade tissue(LPMT/LT)(P<0.05).Principal component analysis results showed that the stress resistance of the leaf anatomical structure of the five species of Rhododendron L.could be assessed using lower epidermal thickness(LLET),LSMT,LPMT/LT,palisade tissue and spongy tissue ratio(LPMT/SMT),and protrusion of the main vein(LMPD).The stress resistance of the five species of Rhododendron L.was arranged as follows:Rhododendron nivale(RNH)>Rhododendron wardii(RWW)>Rhododendron bulu(RBH)>Rhododendron nyingchiense(RNR)>Rhododendron aganniphum(RAB).In summary,RNH shows strong resistance and can be utilized in the selection of Rhododendron varieties.

关 键 词：杜鹃花属 解剖结构 抗逆性评价 色季拉山 

分 类 号：S685.21[农业科学—观赏园艺]