127例疑似细菌性腹泻者粪便标本中病原微生物检测及药敏试验结果调查  

The Detection of Pathogenic Microorganismsand Drug Susceptibility Test in Stool Samples of 127 Cases of Bacterial Diarrhea

作　　者：赖宇尧 李桂珊 刘沿 王志明 LAI Yuyao;LI Guishan;LIU Yan;WANG Zhiming(Nan’ao People’s Hospital Dapeng New District,Shenzhen 518108,China)

机构地区：[1]深圳市大鹏新区南澳人民医院检验科,广东深圳518108

出　　处：《标记免疫分析与临床》2024年第5期897-902,共6页Labeled Immunoassays and Clinical Medicine

摘　　要：目的探讨分析疑似细菌性腹泻者粪便标本中病原微生物检测及药敏试验结果。方法在我院开展为期1年的调查,选取2023年1月至2023年10月我院收治的127例疑似细菌性腹泻患者,采集粪便标本8mL进行病原微生物检测,记录菌株检出情况及分布情况,并采用微生物药敏试纸扩散法行药敏试验分析病原菌的耐药性。结果(1)127例疑似细菌性腹泻患者中,进行细菌初筛125例,其中阴性82例,阳性43例,包括副溶血弧菌7例,沙门菌11例,沙门菌并其他细菌3例,致病性大肠埃希菌2例,致病性大肠埃希菌并其他细菌1例,其他细菌19例。后续经区、市确认结果有一定出入,市确诊结果最终经复核无误,共计检出菌株42株。(2)127例疑似细菌性腹泻患者剔除24例空白,病毒检出阴性64例,阳性39例,共计检出病毒41株。(3)季节分布结果显示,6~8月细菌检出率最高,3~5月次之;9~10月病毒检出率最高,1~2月次之,不同季节细菌、病毒检出率差异有统计学意义(P<0.05)。(4)不同性别细菌、病毒检出率对比差异无统计学意义(P>0.05)。(5)年龄阶段分布结果显示,细菌、病毒均在儿童及老年群体中检出率较高,不同年龄阶段细菌、病毒检出率差异有统计学意义(P<0.05)。(6)药敏试验结果显示,沙门菌对环丙沙星耐药率最高,达50.00%,其次是头孢他啶为42.86%;副溶血弧菌对头孢唑林耐药率最高,达71.43%,其次为头孢他啶、头孢曲松、庆大霉素、头孢呋辛,均为57.14%;致病性大肠埃希菌对环丙沙星耐药率最高,达100.00%;其他细菌对头孢呋辛、头孢唑林耐药率最高,达30.00%,其次是庆大霉素为25.00%。结论通过检测发现127例疑似细菌性腹泻患者中,细菌以沙门菌、病毒以诺如病毒较为多见,且细菌感染多发夏季、病毒感染多发秋冬季,以儿童及老年为主要发病群体,临床应加以重视,针对细菌性腹泻可结合药敏试验给予敏感药物治疗以增强疗�Objective To investigate the detection performance of pathogenic microorganisms and the results of drug sensitivity test in stool samples of suspected bacterial diarrhea.Methods A 1-year investigation was carried out in our hospital for this study.127 patients with suspected bacterial diarrhea admitted to our hospital from January,2023 to October,2023 were selected.8mL stool samples were collected for pathogen detection,and the detection and distribution of strains were recorded.The drug susceptibility test was performed to evaluate the drug resistance of pathogenic bacteria by the diffusion method with microbial susceptibility test paper.Results(1)Of 127 suspected bacterial diarrhea patients,125 cases were preliminarily screened for bacteria,of which 82 were negative and 43 were positive,including 7 cases of Vibrio para-hemolyticus,11 cases of salmonella,3 cases of salmonella and other bacteria,2 cases of pathogenic Escherichia coli,1 case of pathogenic Escherichia coli and other bacteria,19 cases of other bacteria.There were certain differences between the confirmed results of the district and the city,and the confirmed results of the city were correct after final review.With these steps,a total of 42 strains were detected.(2)Of 127 suspected bacterial diarrhea patients,24 were excluded,64 were negative,39 were positive,and a total of 41 strains of virus were detected.(3)The results of seasonal distribution showed that the bacterial detection rate was the highest from June to August,followed by March to May.The virus detection rate was the highest from September to October,followed by January to February,and there were significant differences in the detection rates of bacteria and viruses in different seasons(P<0.05).(4)There were no significant differences in the detection rates of bacteria and viruses between different genders(P>0.05).(5)Age distribution results showed that the detection rates of bacteria and viruses were higher in children and elderly groups,and the detection rates of bacteria and viruses we

关 键 词：细菌性腹泻 粪便标本 病原微生物 药敏试验 

分 类 号：R442.2[医药卫生—诊断学]