pH值、温度及培养基对爱德华氏菌生长的影响  

Effect of pH, Temperature and Medium on the Growthof Edwardsiella spp.

作　　者：陈子强 夏理海 李雪贤 刘岚湘 陆娟娟 皮杰 CHEN Ziqiang;XIA Lihai;LI Xuexian;LIU Lanxiang;LU Juanjuan;PI Jie(Hunan Applied Technology University,Changde,Hunan 415100,China;Songzi Animal Husbandry and Veterinary Service Center,Songzi,Hunan 434200,China)

机构地区：[1]湖南应用技术学院,湖南常德415100 [2]松滋市畜牧兽医服务中心,湖北松滋434200

出　　处：《水产养殖》2024年第7期1-5,43,共6页Journal of Aquaculture

基　　金：湖南省高校思想政治工作精品项目(21JP018);湖南省教育厅科学研究项目(22C1200);2022年湖南应用技术学院校级教学研究改革项目(47);湖南省水产养殖类专业校企合作创新创业教育基地(82);湖南省省级线下一流本科课程(346);湖南应用技术学院水产养殖重点建设学科[XKJS(HY2017)-1]。

摘　　要：为探究爱德华氏菌在自然环境下暴发的水质理化参数,设定不同的培养基[胰蛋白胨大豆肉汤(TSB)培养基、溶菌肉汤(LB)培养基]、pH值(5.5,7.0,8.5)及温度(25℃,28℃,32℃),对爱德华氏菌进行培养。结果表明,TSB培养基比LB培养基更适合爱德华氏菌生长;爱德华氏菌在pH值8.5的生长速度高于pH值7.0和pH值5.5,该菌适合在碱性条件下生长;在25℃时,爱德华氏菌生长速度最快,28℃次之,32℃生长速度最慢。指出,爱德华氏菌适合在低盐度、高无机盐、偏碱性、低温环境中生长。提出,在现实养殖过程中,可以通过提高水体盐度、降低pH值和适当调高水温且不影响水产动物正常生长的措施,以降低水产经济动物感染爱德华氏菌发病率,从而达到绿色健康养殖的目的。In order to investigate the physicochemical parameters of water quality for the outbreak of Edwardsiella spp.in the natural environment,the present study was carried out to cultivate Edwardsiella by setting different media(TSB medium,LB medium),pH(5.5,7.0,8.5)and temperature(25,28,32℃).The results showed that TSB medium was more suitable for the growth of Edwardsiella than LB medium.The growth rate of Edwardsiella at pH=8.5 was higher than that at pH=7.0 and pH=5.5,and the bacterium was suitable for growth under alkaline conditions.The fastest growth rate of Edwardsiella was observed at 25℃,followed by 28℃,and the slowest growth was observed at 32℃.In summary,Edwardsiella is suitable for growth in low salinity,high inorganic salt,alkaline and low temperature environment.In the real aquaculture process,it is possible to reduce the morbidity of aquatic economic species infected with Edwardsiella by increasing the salinity,lowering the pH value and appropriately adjusting the water temperature without affecting the normal growth of aquatic animals in order to achieve the green and healthy aquaculture effect.

关 键 词：爱德华氏菌 PH值 温度 培养基 

分 类 号：S963.7[农业科学—水产养殖]