秀珍菇病害种类调查和病原菌鉴定  

Disease Type Investigation and Pathogens Identification of Pleurotus pulmonarius

作　　者：陆娜[1] 王伟科[1] 林佳瑶 LU Na;WANG Weike;LIN Jiayao(Hangzhou Academy of Agricultural Sciences,Hangzhou 310024,China)

机构地区：[1]杭州市农业科学研究院,浙江杭州310024

出　　处：《中国食用菌》2024年第3期107-120,共14页Edible Fungi of China

基　　金：浙江省农业新品种选育重大科技专项(2021C02073);国家食用菌产业技术体系(CARS-20)。

摘　　要：近年来,病害频发对秀珍菇产量和品质产生了严重的影响,对其病原菌的鉴定和特性研究非常重要。此次采集了不同时期的患病秀珍菇样本,通过分离纯化获得病原菌,采用科赫法则、显微特征、ITS或16S rRNA测序等方法进行病原菌鉴定。结果表明,真菌病原菌主要有木霉属、青霉属、链孢霉属、毛霉属和毛壳菌属菌类,引发绿霉病、红粉病等真菌性病害,多发生于秀珍菇营养期。细菌病原菌主要有假单胞菌属、欧文菌属、伯克氏菌属、窄食单胞菌属及噬几丁质菌属菌类,引发黄菇病、枯萎病等细菌性病害,多发生于秀珍菇生殖生长期。In recent years,the frequent occurrence of diseases has caused a serious impact on the yield and quality of Pleurotus pulmonarius,so it is very important to identify and study the characteristics of the pathogens.Pathogens were isolated and purified from diseased samples collected in different periods.Koch's rule,microscopic characteristics,ITS or 16S rRNA sequencing were used to identify the pathogens.The results showed that the main fungal pathogens were Trichoderma,Penicillium,Streptospora,Mucor,Chaetomium,which caused green mold disease,red powder disease and so on.Fungal diseases mostly occurred in the vegetative stage of P.pulmonarius.Bacterial pathogens mainly include Pseudomonas,Ewingella,Burkholderia,Stenotrophomonas and Chitinophaga,which caused yellow mushroom disease and wilt disease.Bacterial diseases mostly occur in the reproductive growth period of P.pulmonarius.

关 键 词：秀珍菇 真菌 细菌 分子鉴定 

分 类 号：S646.1[农业科学—蔬菜学]