RT-F2000在妇科泌尿生殖道病原微生物检测中的诊断与应用  

Diagnosis and Application of RT-F2000 in Gynecological Urogenital Tract Pathogenic Microorganisms Detection

作　　者：袁子程 YUAN Zicheng(Laboratory Department,Tianzhu County People’s Hospital,Qiandong,Guizhou 556699,China)

机构地区：[1]天柱县人民医院检验科,贵州黔东556699

出　　处：《生物医学工程学进展》2024年第2期162-168,共7页Progress in Biomedical Engineering

摘　　要：目的探究在妇科泌尿生殖道病原微生物检测诊断中使用RT-F2000(全自动阴道分泌物检测仪)。方法回顾性分析在天柱县人民医院就诊并开展妇科检查的120例受检者临床资料,样本选取时段为2023年1—11月。全部受检者入院后采集生殖道分泌物标本及尿道标本,共120份样本,使用RT-F2000与微生物培养法分别进行泌尿生殖道病原微生物检测。对比两种检测方式的病原微生物检出情况;以微生物培养法为“金标准”,评价RT-F2000检测诊断效能。结果(1)根据120例分泌物标本检测结果可知,使用微生物培养法共检出阳性标本83例,阳性率69.16%;支原体、霉菌、线索细胞、滴虫分别检出34例(40.96%)、25例(30.12%)、14例(16.87%)、10例(12.05%)。使用RT-F2000共检出阳性标本85例,阳性率70.83%;支原体、霉菌、线索细胞、滴虫分别检出35例(41.17%)、25例(29.41%)、15例(17.64%)、10例(11.76%)。两种检查方式检出的阳性率差异不具有统计学意义(P<0.05)。(2)使用微生物培养法检出的83例阳性标本中,单纯病原体感染、混合病原体感染分别为70例、13例,构成比分别为84.34%、15.66%。使用RT-F2000检出的85例阳性标本中,单纯病原体感染、混合病原体感染分别为72例、13例,构成比分别为84.71%、15.29%。(3)与微生物培养法相比,RT-F2000检测支原体符合率为99.17%,检测霉菌符合率为100.00%,检测线索细胞符合率为99.17%,检测滴虫符合率为100.00%。(4)RT-F2000检测支原体Kappa值为0.961,检测霉菌Kappa值为0.938,检测线索细胞Kappa值为0.952,检测滴虫Kappa值为0.974,与微生物培养法有良好的一致性(P>0.05)。结论将RT-F2000应用于妇科泌尿生殖道病原微生物检测,可对患者生殖道病原体实现快速、精准的诊断,体现出了较高的临床推广应用价值。Objective To explore the application of RT-F2000(automatic vaginal secretions detector)in the detection and diagnosis of pathogenic microorganisms in gynecological urogenital tract.Methods The clinical data of 120 patients who underwent gynecological examination in Tianzhu County People’s Hospital were retrospectively analyzed,and the samples were selected from January 2023 to November 2023.After admission,a total of 120 samples of genital tract secretions and urethra samples were collected.RT-F2000 and microbial culture method were used to detect pathogenic microorganisms in the genitourinary tract.The detection of pathogenic microorganisms was compared between RT-F2000 and microbial culture method.Microbial culture method was used as the gold standard to evaluate the diagnostic efficacy of RT-F2000.Results(1)The results of 120 samples of secretions showed that 83 positive specimens were detected by microbial culture method,with a positive rate of 69.16%.There were 34 cases(40.96%)of mycoplasma,25 cases(30.12%)of mold,14 cases(16.87%)of cue cells and 10 cases(12.05%)of trichomonas,respectively.The positive rate of RT-F2000 was 70.83%(85 cases).There were 35 cases(41.17%)of mycoplasma,25 cases(29.41%)of mold,15 cases(17.64%)of cue cells and 10 cases(11.76%)of trichomonas,respectively.There was no statistical significance in the positive rate between the two methods(P<0.05).(2)Of the 83 positive specimens by microbial culture method,70 were infected with simple pathogens and 13 were infected with mixed pathogens,respectively,with a composition ratio of 84.34%and 15.66%.In the 85 positive samples by RT-F2000,72 cases were infected with simple pathogens and 13 cases were infected with mixed pathogens,respectively,with a composition ratio of 84.71%and 15.29%.(3)Compared with the microbial culture method,the coincidence rate of RT-F2000 detection of mycoplasma was 99.17%;The coincidence rate of mold was 100.00%.The cue cell coincidence rate was 99.17%.The coincidence rate of trichomonas was 100.00%.(4)The Kappa valu

关 键 词：妇科 泌尿生殖道 病原微生物检测 RT-F2000 微生物培养法 

分 类 号：R446.1[医药卫生—诊断学]