基于Notch信号通路抑制剂的多功能血管支架涂层制备及表征  

Preparation and Characterization of Multifunctional Vascular Stent Coating Based on Notch Signal Pathway Inhibitor

作　　者：金磊源 胡芳坤 姜晓娇 夏立 刘冉 徐佳乐 涂秋芬[1,2] 熊开琴[2] JIN Leiyuan;HU Fangkun;JIANG Xiaojiao;XIA Li;LIU Ran;XU Jiale;TU Qiufen;XIONG Kaiqin(Key Laboratory of Advanced Technologies of Materials of Ministry of Education,College of Materials Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China;Institute of Biomedical Engineering,College of Medicine,Southwest Jiaotong University,Chengdu 610031,China)

机构地区：[1]西南交通大学材料科学与工程学院,材料先进技术教育部重点实验室,成都610031 [2]西南交通大学医学院,生物医学工程研究院,成都610031

出　　处：《材料导报》2024年第12期44-51,共8页Materials Reports

摘　　要：炎症反应贯穿动脉粥样硬化(AS)全过程,且与AS病变部位血管支架植入后的一系列不良事件高度相关,包括支架内再狭窄(ISR)、晚期血栓(LST)以及支架内新生动脉粥样硬化(ISNA)等。在诸多炎性疾病(包括AS)的发生及进展过程中,Notch信号通路均被异常激活。因此,Notch信号通路抑制剂(NSPI)的装载有望减轻炎症,进而缓解支架植入并发症。基于此,本工作提出了以聚乳酸-羟基乙酸共聚物(PLGA)为载体,采用超声雾化喷涂法将Notch信号通路抑制剂RO4929097装载于支架表面的改性策略,构建NSPI/PLGA多功能血管支架。研究结果表明,NSPI/PLGA血管支架的RO4929097有效释放长达30 d,可同时实现抑制炎症反应、促进内皮细胞增殖和抗平滑肌细胞增殖以及抗凝血等多种功能,在介入治疗领域具有极大的应用潜力。Inflammatory reaction runs through the whole process of atherosclerosis(AS),and is highly related to a series of adverse events after vascular stent implantation,including in stent restenosis(ISR),late stent thrombosis(LST)and in stent neoatherosclerosis(ISNA)and so on.In many inflammatory diseases(including AS),Notch signaling pathway is abnormally activated.Therefore,the loading of Notch signaling pathway inhibitor(NSPI)is expected to reduce inflammation and then alleviate the complications of stent implantation.Herein,we proposed a modification strategy,using poly(lactic acid glycolic acid)copolymer(PLGA)as the carrier to load RO4929097,Notch signal pathway inhibitor,the surface of stent by ultrasonic atomization spraying to construct the NSPI/PLGA multi-functional vascular stent.The results show that NSPI/PLGA vascular stent can effectively release RO4929097 for up to 30 d,and can simultaneously inhibit inflammation,promote endothelial cell proliferation,anti-smooth muscle cell proliferation and anticoagulation.So this strategy has great potential in the field of interventional therapy.

关 键 词：动脉粥样硬化 血管支架 炎症 Notch信号通路抑制剂 超声雾化喷涂技术 表面改性 

分 类 号：O647[理学—物理化学]