基于丙烯酸-N-琥珀酰亚胺酯共聚物交联剂的壳聚糖水凝胶的生物相容性研究  

Biocompatibility of Chitosan Hydrogels Based on Acrylic-N-Succinimidyl Ester Copolymer Crosslinker

作　　者：杨水艳 盛扬 孙一新 蔡仁钦 Mark Bradley 张嵘 YANG Shuiyan;SHENG Yang;SUN Yixin;CAI Renqin;Mark Bradley;ZHANG Rong(School of Materials Science and Engineering,Changzhou University,Changzhou 213164,Jiangsu,China;Basic Research Institute,Double Medical Technology Inc.,Xiamen 361026,Fujian,China;School of Chemistry,University of Edinburgh,Scotland EH93JJ,Edinburgh,UK)

机构地区：[1]常州大学材料科学与工程学院,江苏常州213164 [2]大博医疗科技有限公司基础研究院,福建厦门361026 [3]英国爱丁堡大学化学学院,英国爱丁堡EH93JJ

出　　处：《材料导报》2024年第12期234-243,共10页Materials Reports

基　　金：江苏省六大人才高峰创新团队(SWYY-CXTD-001);常州市科技局国际合作项目(CZ20190019)。

摘　　要：壳聚糖作为一种天然的氨基多糖,拥有良好的生物相容性和资源可再生性,但是较差的力学性能限制了其更广泛的应用。本研究以丙烯酸、N-乙烯基吡咯烷酮和丙烯酸-N-琥珀酰亚胺酯为单体聚合得到三元共聚物大分子交联剂,然后将其与壳聚糖溶液混合后制备壳聚糖水凝胶。采用红外、热重、SEM等对合成的聚合物及壳聚糖凝胶的组成、形貌和热稳定性进行了表征。结果表明,丙烯酸-N-琥珀酰亚胺酯共聚物的数均分子量为111900,具有一定的水溶性,与壳聚糖形成的水凝胶有良好的力学性能、溶胀性能和水蒸气渗透速率。在PBS(pH=7.4)中交联的凝胶膜与酸性条件下制备的凝胶膜相比,热分解温度提高,水接触角变大,力学性能得到较大提升。细胞毒性实验表明壳聚糖凝胶有良好的生物相容性,人脂肪干细胞在壳聚糖凝胶膜上会形成细胞簇并分泌外泌体。将壳聚糖凝胶膜覆盖在脂肪干细胞上,细胞能正常生长并分泌外泌体。因此,壳聚糖凝胶具有负载外泌体后应用于皮肤敷料的潜力。As a natural amino polysaccharide,chitosan has good biocompatibility and renewable resources,but its application is limited due to poor mechanical properties.In this study,acrylic acid,N-vinylpyrrolidone and acrylic acid N-succinimide ester were used as monomers to obtain terpolymer macromolecular crosslinker,which was mixed with chitosan solution to prepare chitosan hydrogel.The composition,morphology and thermal stability of the synthesized polymer and chitosan gel were characterized by infrared,thermogravimetric analysis and SEM.The results showed that the average molecular weight of acrylate-N-succinimide ester copolymer was 111900,which was water soluble.The chitosan hydrogel with the copolymer crosslinker showed good mechanical properties,swelling properties and water vapor permeation rate.Compared with the gel membrane prepared under acidic conditions,the thermal decomposition temperature of the crosslinked gel membrane in PBS(pH=7.4)increased,the contact angle of water became larger,and the mechanical properties were greatly improved.Cytotoxicity experiments had shown that chitosan gels have good biocompatibility.Human adipose-derived stem cells formed cell clusters on the gels and secreted exosomes on the chitosan gel membrane.By covering the chitosan gel membrane over the stem cells,the cells grew normally and secreted exosomes.Chitosan gels therefore have the potential to be applied to skin dressings after loading exosomes.

关 键 词：壳聚糖 丙烯酸-N-琥珀酰亚胺酯 水凝胶 外泌体 

分 类 号：TQ460.4[化学工程—制药化工]