广粪蚊共生菌发酵液对宿主嗅觉行为反应的影响及其挥发物成分分析  

Effects of Fermentation Broths of Symbiotic Bacteria in Coboldia fuscipes(Meigen)on Host Olfactory Behavioral Responses and Analysis of Volatiles

作　　者：李炳宏 王剑峰 王丹丹 LI Binghong;WANG Jianfeng;WANG Dandan(Liaoning Key Laboratory of Urban Integrated Pest Management and Ecological Security,College of Life Science and Bioengineering,Shenyang University,Shenyang 110044,Liaoning,China)

机构地区：[1]沈阳大学生命科学与工程学院辽宁省城市有害生物治理与生态安全重点实验室,中国辽宁沈阳110044

出　　处：《生命科学研究》2024年第3期232-240,共9页Life Science Research

基　　金：国家自然科学基金资助项目(31672332);辽宁省自然科学基金项目(2019-ZD-0539)。

摘　　要：广粪蚊[Coboldia fuscipes (Meigen)]是食用菌的主要害虫之一,昆虫共生菌潜在的生防价值为广粪蚊的绿色防治提供了新的思路。本研究首先使用Y型嗅觉仪测试了广粪蚊成虫对其14科16属21种共生菌发酵液在10 min、20 min、30 min 3个测试时间段内的嗅觉行为反应。结果表明,水生产碱菌(Alcaligenes aquatilis)、柠檬酸杆菌未知种(Citrobacter sp.)、格陵兰变色杆菌(Paenochrobactrum glaciei) 3种共生菌的发酵液对广粪蚊成虫具有显著的引诱作用,沙福芽孢杆菌(Bacillus safensis)发酵液对广粪蚊成虫具有显著的趋避作用,其30 min时的相对引诱率分别达到了20.00%、13.75%、15.00%和-18.75%。随后,基于气相色谱-质谱联用仪(gas chromatography-mass spectrometry, GC-MS)分析了上述4种发酵液的挥发物。结果显示,共鉴定出相对含量高于2%的化合物28种,水生产碱菌与格陵兰变色杆菌发酵液中相对含量最高的挥发物是苯甲醛,其次分别是2,5-二甲基吡嗪和十二烷二腈;柠檬酸杆菌未知种发酵液中相对含量最高的挥发物是N-苯基甲酰胺,其次是异戊酸异戊酯;沙福芽孢杆菌发酵液中相对含量最高的挥发物是异戊酸乙酯,其次是异戊酸异丙酯。本研究明确了发酵液对广粪蚊成虫具有引诱与趋避作用的共生菌,并且测定了发酵液挥发物的具体成分,为开发广粪蚊引诱剂或驱避剂提供了依据。Coboldia fuscipes(Meigen)is one of the main pests of edible fungi.The symbiotic bacteria in the insect may have a potential biocontrol value and be used in green control of the insect.Herein,the Y-type olfactory tester was used to test the olfactory behavior responses of the C.fuscipes adults to the fermentation broths of 21 symbiotic bacteria,which belong to 16 genera in 14 families,in 3 test periods of 10 min,20 min and 30 min.The results showed that the fermentation broths of Alcaligenes aquatilis,Citrobacter sp.and Paenochrobactrum glaciei had a significant attractant effect on the adults,while the fermentation broth of Bacillus safensis had a significant repellent effect on the adults.The relative attractive rates of these four broths reached 20.00%,13.75%,15.00%and-18.75%in 30 min,respectively.Then the volatiles of the four fermentation broths were analyzed by gas chromatography-mass spectrometry(GC-MS).The results showed that 28 compounds with the relative content higher than 2%were identified.In both A.aquatilis and P.glaciei broths,benzaldehyde is the most abundant volatile,followed by pyrazine,2,5-dimethyl-and 1,10-dicyanodecane,respectively.The relative content of formamide,N-phenyl-was the highest in C.sp.,followed by butanoic acid,3-methyl-,3-methylbutyl ester.The relative content of butanoic acid,3-methyl-,ethyl ester was the highest in B.safensis,followed by butanoic acid,3-methyl-,1-methylethyl ester.In conclusion,the symbiotic bacteria with attractant or repellent effect on adult C.fuscipes were identified,and specific components in the volatiles were determined,which would benefit the develop-ment of attractants or repellents for C.fuscipes.

关 键 词：广粪蚊 共生菌 Y型嗅觉测试仪 嗅觉行为 气相色谱-质谱联用仪(GC-MS) 挥发物成分分析 

分 类 号：Q939.9[生物学—微生物学] Q969.9