低表达ACOT4促进草酸钙对HK2细胞损伤和结晶形成  

Low-expression of ACOT4 Promotes the Damage and Crystals Formation of HK2 Cell by Calcium Oxalate

作　　者：王升晗 雷振涛[1] 史玉强[1] 高强[1] 张保[1] WANG Shenghan;LEI Zhentao;SHI Yuqiang(Department of Urology,Aerospace Center Hospital,Beijing 100049,China)

机构地区：[1]航天中心医院泌尿外科,北京100049

出　　处：《医学研究杂志》2024年第6期113-118,共6页Journal of Medical Research

基　　金：吴阶平医学基金会临床科研专项基金资助项目(320.6750.19085)。

摘　　要：目的探讨酰基辅酶A硫代酯酶4(acyl-CoA thioeaterase 4,ACOT 4)的表达对草酸钙结石形成的影响。方法以人肾小管上皮细胞HK2细胞为研究对象,使用草酸钙处理HK2细胞,并使用siRNA干扰HK2细胞中ACOT4的表达。qPCR和Western blot法检测HK2细胞中基因的表达水平;CCK-8法检测细胞活率;流式细胞术检测细胞凋亡情况;LDH实验检测细胞损伤情况;晶体黏附实验检测HK2细胞对草酸钙结晶的黏附能力。结果草酸钙可以调节HK2细胞中ACOT4的表达;干扰ACOT4可以显著抑制HK2细胞的增殖能力,并且增强草酸钙对HK2细胞的活性降低、损伤和凋亡的影响;同时,干扰ACOT4可以显著促进HK2细胞对草酸钙晶体的黏附能力。结论敲低ACOT4可以促进草酸钙对HK2细胞的损伤并促进HK2细胞对草酸钙晶体的黏附能力。Objective To investigate the effect of ACOT4 expression on the formation of calcium oxalate stones.Methods The HK2 cell of human tubular epithelial cells was used as subject,HK2 cell was treated with calcium oxalate,and the expression of ACOT4 was interfered with by siRNA.The gene expression levels in HK2 cell were detected by qPCR and Western blot.The cell viability was detected by CCK-8 assay.The cell apoptosis was detected by flow cytometry.The cell damage was detected by LDH assay.The adhesion ability of HK2 cell to calcium oxalate crystals was detected by crystal adhesion experiment.Results Calcium oxalate could regulate the expression of ACOT4 in HK2 cell.Interfering with ACOT4 can significantly inhibit the proliferation ability of HK2 cell,and promote the effect of cell activity reduction,damage and apoptosis of calcium oxalate to HK2 cell.At the same time,interfering with ACOT4 can significantly promote the adhesion ability of HK2 cells to calcium oxalate crystals.Conclusion Knocking down of ACOT4 can promote the damage of calcium oxalate to HK2 cell and promote the adhesion ability of HK2 cell to calcium oxalate crystals.

关 键 词：肾结石 草酸钙 ACOT4 HK2细胞 

分 类 号：R692.5[医药卫生—泌尿科学]