重组禽流感病毒(H5+H7)三价灭活疫苗免疫黄鸡抗体水平分析  

Immune antibody level analysis of rAIV(H5+H7)in yellow chickens

作　　者：黄青 杨建德 刘燕霏 Huang Qing;Yang Jiande;Liu Yanfei(Tianjin Key Laboratory of Agricultural Animal Breeding and Healthy Husbandry,College of Animal Science and Veterinary Medicine,Tianjin Agricultural University,Tianjin 300392,China)

机构地区：[1]天津农学院动物科学与动物医学学院天津市农业动物繁育与健康养殖重点实验室,天津300392

出　　处：《天津农学院学报》2024年第3期59-62,共4页Journal of Tianjin Agricultural University

基　　金：天津市自然科学基金(07BJC16000)。

摘　　要：H5或H7亚型高致病性禽流感是一种高度致死性传染病,它不仅给养禽业造成了巨大的危害和损失,还威胁着人类的生命健康安全。为了分析重组禽流感病毒(H5+H7)三价灭活疫苗(H5N1 Re-11株+H5N1 Re-12株和H7N9 H7-Re3株)免疫抗体水平,本研究对如皋市内14个养殖场近2万只黄鸡进行重组禽流感病毒三价灭活疫苗肌肉注射,免疫三周后,随机采集420份血液样品进行HI抗体效价检测。结果表明,免疫后的鸡群均无明显不良反应,可以诱导黄鸡产生良好的抗体,检测时具有较高的抗体效价,H5亚型Re-11株平均抗体效价为7.8log2,Re-12株平均抗体效价为8.2log2,H7亚型Re-3株的平均抗体效价为6.8log2,并且免疫抗体合格率均达到70%以上,对黄鸡具有良好的安全性和免疫保护效果,研究结果为有效地控制高致病性禽流感提供参考依据。H5 or H7 subtype highly pathogenic avian influenza is a highly lethal infectious disease.It not only causes great harm and loss to the poultry industry,but also threatens the safety of human life and health.The purpose of this study is to analyze antibody levels of the trivalent inactivated vaccine of recombinant avian influenza virus(H5+H7)based on H5N1 Re-11 strain+Re-12 strain and H7N9 Re-3 strain.In this study,nearly 20000 yellow chickens from 14 farms in Rugao city were intramuscularly injected with recombinant avian influenza virus trivalent inactivated vaccine.After three weeks of immunization,420 blood samples were randomly collected for HI antibody titer detection.The results showed that there were no obvious adverse reactions in all the vaccinated chickens,and the yellow chickens could be induced to produce good antibodies with high antibody titers.The average antibody titer of H5 subtype Re-11 strain and Re-12 strain were 7.8log2 and 8.2log2 respectively.The average antibody titer of H7 subtype Re-3 strain was 6.81og2,and the pass rate of immune antibody was more than 70%.It had good safety and immune protection effect on yellow chickens.The results provide a reference basis for the effective control of highly pathogenic avian influenza.

关 键 词：重组禽流感病毒 H5+H7亚型 三价灭活疫苗 免疫 抗体水平 

分 类 号：S858.31[农业科学—临床兽医学]