根结线虫病对烟草植株根际土壤微生物群落及其功能的影响  

Effects of root-knot nematode disease on microbial community and function in rhizosphere soil of tobacco plants

作　　者：王兴松 李恩星 杨诗瀚 杨媛 陈江政 李晗梅 王戈[1] 王娜[1] 白羽祥 周鹏 杜宇 李杰[2] WANG Xingsong;LI Enxing;YANG Shihan;YANG Yuan;CHEN Jiangzheng;LI Hanmei;WANG Ge;WANG Na;BAI Yuxiang;ZHOU Peng;DU Yu;LI Jie(College of Tobacco,Yunnan Agricultural University,Kunming 650201,China;Yunnan Tobacco Company Kunming Branch,Kunming 650051,China)

机构地区：[1]云南农业大学烟草学院,云南昆明650201 [2]云南省烟草公司昆明市公司,云南昆明650051

出　　处：《江苏农业学报》2024年第6期993-1003,共11页Jiangsu Journal of Agricultural Sciences

基　　金：国家自然科学基金项目(32160517);云南省烟草公司昆明市公司科技计划项目[昆烟科(2022)12号KMYC202207]。

摘　　要：为明确烟草植株根际土壤微生物对烟株根结线虫病的响应特征,采用Illumina MiSeq高通量测序技术测定健康烟株(FH-H)、发病烟株(FH-D)根际土壤细菌16S rDNA和真菌内转录间隔区(ITS)序列,测定其根际土壤理化性质和酶活性,并借助PICRUSt2软件和FUNGuild数据库进行分析。结果表明,与健康烟株相比,发病烟株根际土壤pH、硝态氮含量、碱解氮含量、过氧化氢酶活性和微生物群落α多样性显著降低;发病烟株根际土壤微生物种类和相对丰度发生改变,细菌群落中放线菌门、厚壁菌门、芽孢杆菌属和Unclassified_Ktedonobacteraceae属相对丰度均显著升高,真菌群落中子囊菌门、担子菌门、青霉属、粗糙孔菌属相对丰度均显著升高。发病烟株根际土壤细菌群落在碳水化合物代谢、氨基酸代谢、辅助因子和维生素代谢等功能丰度上显著高于健康烟株根际土壤,功能真菌类型的相对丰度明显区别于健康烟株根际土壤,其中腐生营养型功能真菌相对丰度显著高于健康烟株根际土壤。方差分解分析结果表明,土壤有效磷含量对烟株根际土壤细菌相对丰度影响最大,烟株根际土壤真菌相对丰度主要与土壤脲酶活性、土壤硝态氮含量和土壤酸碱度有显著相关性。本研究结果为根结线虫病的绿色防控和土壤保育提供了理论依据。In order to clarify the response characteristics of rhizosphere soil microorganisms of tobacco plants to its root-knot nematode disease,Illumina MiSeq high-throughput sequencing technology was used to determine the bacterial 16S rDNA and fungal internal transcribed spacer(ITS)sequences in the rhizosphere soil of healthy tobacco plants(FH-H)and diseased tobacco plants(FH-D).The physicochemical properties and enzyme activities of the rhizosphere soil were determined,and PICRUSt2 software and FUNGuild database analysis were performed.The results showed that compared with healthy tobacco plants,the pH,nitrate nitrogen content,alkali-hydrolyzable nitrogen content,catalase activity and microbial communityαdiversity of rhizosphere soil of diseased tobacco plants decreased significantly.The microbial species and relative abundance changed.The relative abundances of Actinobacteria,Firmicutes,Bacillus and Unclassified_Ktedonobacteraceae in the bacterial community increased significantly,and the relative abundances of Ascomycota,Basidiomycota,Penicillium and Trechispora in the fungal community increased significantly.The abundances of carbohydrate metabolism,amino acid metabolism,cofactors and vitamin metabolism of the bacterial community in the rhizosphere soil of diseased tobacco plants were significantly higher than those in the rhizosphere soil of healthy tobacco plants.The relative abundance of functional fungal types in the rhizosphere soil of diseased tobacco plants was obviously different from that of healthy tobacco plants.The relative abundance of saprophytic functional fungi in the rhizosphere soil of diseased tobacco plants was significantly higher than that of healthy tobacco plants.The results of variance decomposition analysis showed that the soil available phosphorus content had the greatest influence on the bacterial relative abundance in the rhizosphere soil of tobacco plants.The fungal relative abundance in the rhizosphere soil of tobacco plants was mainly correlated with soil urease activity,soil nitrat

关 键 词：根结线虫 烟草 根际土壤 微生物群落 微生物功能 

分 类 号：S572.04[农业科学—烟草工业]