转基因定量检测量值转换系数的研究进展  

Research Progress on the Conversion Factor of Quantity Value for Genetically Modified Organism Quantification

作　　者：刘圆圆 李佳丽 吉帅帅 高运华[3] 刘旦梅[1] 王迪 LIU Yuanyuan;LI Jiali;JI Shuaishuai;GAO Yunhua;LIU Danmei;WANG Di(College of Life Sciences,Shanxi University,Taiyuan,Shanxi 030000,China;College of Pharmacy and Bioengineering,Chongqing University of Technology,Chongqing 400054,China;Center for Advanced Measurement Science,National Institute of Metrology,Beijing 100029,China)

机构地区：[1]山西大学生命科学学院,山西太原030000 [2]重庆理工大学药学与生物工程学院,重庆400054 [3]中国计量科学研究院前沿计量科学中心,北京100029

出　　处：《计量学报》2024年第6期922-928,共7页Acta Metrologica Sinica

基　　金：国家农业生物育种重大项目(2022ZD04020)。

摘　　要：基于PCR技术测定的转基因成分定量结果主要是拷贝数比值,而“产品标识”规定检测结果必须以质量分数表示。量值单位的不统一,造成无法根据检测结果直接进行标识与否的判定。因此亟须研究转基因含量2种单位间的量值关系,建立实现量值转换的“转换系数”定值方法,从而保障检测结果与标识阈值的直接可比。通过对国际转基因“产品标识”制度进行总结,系统分析了影响“转换系数”量值的关键因素和欧盟建立的定值方法,并就我国开展相关研究的重要性进行了论述,为我国转基因“产品标识”制度的有效执行提供参考。The quantitative results of GM content determined by PCR-based technology are mainly copy number ratio,whereas the labeling regulation specified that the quantitative result must be expressed in the form of a mass fraction.The lack of consistency in measurement unit hinders direct decision-making based on test results.It is urgent to investigate the quantity value relationship between the two measurement scales,and establish a methodology for determining the“conversion factor”value,thus ensuring the direct comparison between quantitative results and labeling threshold.The international labeling systems of genetically modified product are summarized,key affect factors for determining the“conversion factor”and characterization method established by the European Union are systematically analyzed,and the importance of conducting the relevant study in China is also discussed.This review can provide reference for the effective implementation of the labeling system in China.

关 键 词：生物计量 转基因产品 标准物质 转换系数 数字PCR 产品标识制度 

分 类 号：TB99[一般工业技术—计量学]