UPLC-MSMS法测定酸枣仁γ-氨基丁酸颗粒剂中酸枣仁皂苷A和B含量  

Determination of Jujube Saponin A(JuA)&Jujube Saponin B(JuB)in ZSS/γ-aminobutyric Acid Granules by UPLC-MSMS Method

作　　者：黄昉 陈慧 HUANG Fang;CHEN Hui(Guangzhou Branch,SGS-CSTC Standards Technical Services Co.,Ltd.,Guangdong Guangzhou 510663,China)

机构地区：[1]通标标准技术服务有限公司广州分公司,广东广州510663

出　　处：《广州化工》2024年第7期134-138,共5页GuangZhou Chemical Industry

摘　　要：采用色谱柱Waters ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm);流动相为0.1%甲酸溶液-乙腈(68∶32);流速:0.4 mL/min等度洗脱;采用MRM模式,建立一个酸枣仁γ-氨基丁酸颗粒剂中酸枣仁皂苷A和B的UPLC-MSMS测定方法。颗粒剂经超声溶解后测定,在酸枣仁皂苷A和B含量分别为100.2~1002.0μg/L和50.5~505.0μg/L范围内线性系数R均大于0.999,呈良好的线性;各前处理影响因子经摸索后设置为最佳,此时各因子微小变动也能满足系统适用性试验要求,重复性和中间精密度相对标准偏差均小于6%,回收率为分别为101.06%~112.71%和91.80%~102.06%。该方法体现出良好的准确度、精密度、耐用性,适用于酸枣仁γ-氨基丁酸颗粒剂中酸枣仁皂苷A和B的测试。The determination was performed on a Waters ACQUITY UPLC BEH C_(18)(2.1 mm×100 mm,1.7μm)column,with the mobile phase was 0.1%formic acid-acetonitrile(68∶32),at the flow rate of 0.4 mL/min.An UPLC-MSMS method for the determination of Jujube saponin A(JuA)and Jujube saponin B(JuB)in ZSS/γ-aminobutyric acid granules was established by using MRM model.the linear coefficient R of JuA and JuA in the range of 100.2~1002.0μg/L and 50.5~505.0μg/L,respectively,were greater than 0.999,showing a good linearity.The relative standard deviations of repeatability and intermediate precision were less than 6%,and the recoveries were 101.06%~112.71%and 91.80%~102.06%,respectively.This method showed good accuracy,precision and durability,and was suitable for the determination of JuA and JuB in ZSS/γ-aminobutyric acid granules.

关 键 词：UPLC-MSMS法 酸枣仁皂苷A和B 

分 类 号：O657.63[理学—分析化学]