基于WGCNA挖掘天津猴鸡裸颈性状相关基因  

Exploration of genes related to the naked neck trait in Tianjin-monkey chickens based on WGCNA

作　　者：吴鹏飞 夏树立[1,2,3] 于海涛 赵向华[1,2,3] 王康 WU Pengfei;XIA Shuli;YU Haitao;ZHAO Xianghua;WANG Kang(Institute of Animal Science and Veterinary,Tianjin Academy of Agricultural Sciences,Tianjin 300381,China;Tianjin Key Laboratory of Animal Molecular Breeding and Biotechnology,Tianjin 300381,China;Tianjin Engineering Research Center of Animal Healthy Farming,Tianjin 300381,China;College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China)

机构地区：[1]天津市农业科学院畜牧兽医研究所,天津300381 [2]天津市畜禽分子育种与生物技术重点实验室,天津300381 [3]天津市畜禽健康养殖工程技术中心,天津300381 [4]扬州大学动物科学与技术学院,江苏扬州225009

出　　处：《江苏农业学报》2024年第5期881-889,共9页Jiangsu Journal of Agricultural Sciences

基　　金：天津市农业科学院财政种业创新研究项目(2023ZYCX011)。

摘　　要：天津猴鸡是中国珍贵的裸颈鸡遗传资源,为在全基因组范围内探究其裸颈发育相关基因,本研究采集天津猴鸡杂交F_(1)代裸颈鸡和常羽鸡颈部皮肤组织用于转录组测序。加权基因共表达网络分析(WGCNA)结果显示,识别到的10个基因模块中只有蓝绿色(turquoise)和蓝色(blue)基因模块与裸颈表型显著相关,共计583个基因;GO功能富集分析富集到脂滴组织、甘油三酯储存负调控以及肌肉收缩等脂肪和肌肉相关生物学过程等条目;KEGG通路分析也富集到多条脂肪代谢相关通路,包括PPAR信号通路和甘油酯代谢等;蛋白质互作网络分析结果显示ACTN2基因编码的蛋白质的连通性最高,其次是MYL10基因编码的蛋白质,另外,还发现多个密切参与毛囊发育的基因,包括SOX9和PPARGC等。本研究结果将为进一步揭示并完善鸡裸颈的形成和发育奠定基础,同时对天津猴鸡的保护、开发和利用具有重要意义。Tianjin-monkey chicken is a precious genetic resource of naked neck chicken in China.To identify genes related to the naked neck phenotype at the whole-genome level,we collected neck skin tissue from the hybrid F 1 generation of Tianjin-monkey chickens for transcriptome sequencing.The results showed that among the 10 gene modules identified by weighted gene co-expression network analysis(WGCNA),only the turquoise and blue modules were significantly associated with the naked neck phenotype,comprising a total of 583 genes.GO enrichment analysis revealed biological process terms related to fat and muscle,including lipid droplet organization,negative regulation of triglyceride storage and muscle contraction.KEGG pathway analysis also identified multiple pathways related to fat metabolism,such as PPAR signaling pathway and glycerolipid metabolism.Protein-protein interaction network analysis revealed that the protein encoded by ACTN2 had the highest connectivity,followed by the protein encoded by MYL10.Furthermore,several genes closely involved in hair follicle development,such as SOX9 and PPARGC,were also found.The results of this study can lay a foundation for further revealing and improving the formation and development of naked neck in chickens.Moreover,it is of great significance for the protection,development,and utilization of Tianjin-monkey chicken.

关 键 词：天津猴鸡 裸颈性状 加权基因共表达网络分析(WGCNA) 

分 类 号：S831.2[农业科学—畜牧学]