尾梢去除处理对板栗结果枝基部芽激素含量的影响  

Effects of tail tip removal treatments on the content of hormones in the basal buds of chestnut fruiting branches

作　　者：吕梦炀 马亚特 肖丽静 张海娥 齐永顺[1,2,3,4] 张京政 王东升[2,4,5] LYU Mengyang;MA Yate;XIAO Lijing;ZHANG Hai’e;QI Yongshun;ZHANG Jingzheng;WANG Dongsheng(College of Horticultural Science and Technology,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China;Engineering Research Center of Chestnut Industry Technology,Ministry of Education,Hebei Normal University of Science and Technology,Qinhuangdao 066004,China;Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization,Qinhuangdao 066004,China;Hebei Collaborative Innovation Center of Chestnut Industry,Qinhuangdao 066004,China;Hebei Yanshan Characteristic Agricultural Technology Research Institute,Qinhuangdao 066004,China)

机构地区：[1]河北科技师范学院园艺科技学院,河北秦皇岛066004 [2]河北科技师范学院板栗产业技术教育部工程研究中心,河北秦皇岛066004 [3]河北省特色园艺种质挖掘与创新利用重点实验室,河北秦皇岛066004 [4]河北省板栗产业协同创新中心,河北秦皇岛066004 [5]河北省燕山特色农业产业技术研究院,河北秦皇岛066004

出　　处：《江苏农业学报》2024年第5期922-934,共13页Jiangsu Journal of Agricultural Sciences

基　　金：国家重点研发计划项目(2022YFD2200400)。

摘　　要：分析尾梢去除处理对基部芽激素含量的影响,可为了解隐芽萌发条件提供一定的参考。本研究以燕秋为试验材料,分别在花后第1 d(TA,5月25日)、第21 d(TB,6月14日)和第41 d(TC,7月4日)进行尾梢去除处理,并在每次去除尾梢后第5 d、第10 d、第15 d、第20 d取1次基部芽。采用液相色谱串联质谱(LC-MS/MS)技术测定不同处理的板栗结果枝基部芽中的独脚金内酯(SL)含量、赤霉素(GA)含量、生长素(Auxin)含量、细胞分裂素(CTK)含量、脱落酸(ABA)含量及其比值变化。结果表明,对板栗结果枝去除尾梢后均促进了基部芽平均萌发一年生枝条总数;TA处理提高了基部芽平均萌发一年生结果枝占比(16.67%)、一年生枝平均发育枝枝长及结果枝枝粗;TB处理、TC处理后基部芽只萌发出了发育枝。对照基部芽未萌发,仅顶端芽萌发一年生枝,平均为3.0个,其中结果枝占比为20.00%。尾梢去除后SL含量在TA处理下降幅度较大;GA含量在TB处理下降幅度较大;Auxin含量在TA处理下降,TB处理上升幅度较大;CTK含量波动幅度与对照相似,差异不显著;ABA含量在TC处理上升幅度较大,而在TA处理下降。吲哚-3-乙酸(IAA)含量与ABA含量的比值总体下降,其中TC处理下降幅度较大;IAA含量与GA含量的比值在TC处理上升幅度较大,波动较明显;ABA含量与GA含量的比值在TA处理下降,TC处理上升幅度较大。此外,相关性分析结果显示,对照基部芽赤霉素含量和脱落酸含量呈显著正相关(r=0.646),而处理基部芽29种激素间相关性主要集中在细胞分裂素和生长素之间,其中IA(吲哚-3-丙烯酸)含量与IAA-Ala[N-(3-吲哚乙酰基)-L-丙氨酸]、ICA(吲哚-3-甲酸)、TRA(色胺)、cZROG(顺式-玉米核苷-O-糖苷)、ABA-GE(脱落酸葡萄糖酯)、DHZ7G(双氢玉米素-7-糖苷)、ICAld(吲哚-3-甲醛)、tZOG(反式-玉米素-9-B-葡萄糖苷)的含量呈极显著正相关;IPR(异戊烯腺嘌呤核苷)含量、tZR(玉米素核苷Analyzing the effects of tail tip removal treatments on the content of hormones in basal buds can provide a certain reference for understanding the germination conditions of hidden buds.In this study,Yanqiu was used as the experimental material.Tail tip removal treatments were performed on the first day(TA,May 25),21st day(TB,June 14)and 41st day(TC,July 4)after flowering.The basal buds were taken on the 5th,10th,15th,and 20th days after each removal of the tail tip.The contents and ratios of strigolactone(SL),gibberellin(GA),auxin,cytokinin(CTK)and abscisic acid(ABA)in the basal buds of chestnut fruiting branches after different treatments were determined by liquid chromatography tandem mass spectrometry(LC-MS/MS).The results showed that the average number of one-year-old branches germinated from the basal buds was promoted after the removal of tail tip of chestnut fruiting branches.TA treatment increased the ratio of fruiting branches(16.67%),branch length of developing branches and the diameter of fruiting branches.After TB treatment and TC treatment,only developing branches germinated from the basal buds.The basal buds of the control did not germinate,and only the top buds germinated one-year-old branches,with an average of three branches,and the ratio of fruiting branches was 20.00%.The SL content decreased greatly in TA treatment.The content of GA decreased greatly in TB treatment.The content of auxin decreased in TA treatment,and increased greatly in TB treatment.The fluctuation range of CTK content was similar to that of the control,and the difference was not significant.The ABA content increased greatly in TC treatment,but decreased in TA treatment.The ratio of indole-3-acetic acid(IAA)content to ABA content decreased overall,and it decreased greatly in TC treatment.The ratio of IAA content to GA content increased greatly in TC treatment,and the fluctuation was obvious.The ratio of ABA content to GA content decreased in TA treatment,and increased significantly in TC treatment.In addition,correlation anal

关 键 词：板栗 尾梢处理 基部芽 液相色谱串联质谱技术 激素 

分 类 号：S664.2[农业科学—果树学]