基于生信分析放射性肺损伤核心基因及靶向中药筛选  

Core genes of radiation lung injury and screening of targeted traditional Chinese medicine based on bioinformatics analysis

作　　者：李建华 郭婷婷 王志鹏 侯彦婕 秦秀军[1] 李建国[1] LI Jianhua;GUO Tingting;WANG Zhipeng;HOU Yanjie;QIN Xiujun;LI Jianguo(Department of Radiology and Environmental Medicine,China Institute for Radiation Protection,Taiyuan030006,China;Institute of Traditional Chinese Medicine Prescriptions,Shanxi Academy of Traditional Chinese Medicine,Taiyuan030012,China)

机构地区：[1]中国辐射防护研究院放射医学与环境医学研究所,山西太原030006 [2]山西省中医药研究院中药方剂研究所,山西太原030012

出　　处：《安徽大学学报（自然科学版）》2024年第3期102-108,共7页Journal of Anhui University(Natural Science Edition)

基　　金：山西省自然科学基金面上项目(202103021224447);中国辐射防护研究院青年基金资助项目(YQ22000206)。

摘　　要：为了探索放射性肺损伤发病机制及预测潜在靶向治疗的中药,利用生物信息学方法分析放射性肺损伤与正常肺组织的表达差异基因,从GEO数据库选取GSE202586数据集,通过GEO_(2)R分析得出表达差异基因,对表达差异基因进行GO功能富集分析及KEGG信号通路分析,通过STRING数据库构建蛋白互作网络,利用Cytoscape获得核心基因,通过Coremine Medical预测靶向作用于核心基因的中药.结果共获得差异基因113个,其中88个基因表达上调,25个基因表达下调,主要涉及细胞过程、免疫过程及信号的调节,非跨膜蛋白酪氨酸激酶和信号受体的活性等生物过程;主要富集在免疫系统信号通路.蛋白互作分析筛选出8个核心基因包括CD19,CD22,CD52,CD86,LCP2,PAX5,SPN,TNFRSF13C.将筛选整合得到的8个核心基因通过数据库进行靶向中药的筛选,归纳总结3类相关治疗的中药,包括以赤芍、苦蘵、白花蛇钱草、白英为代表的清热解毒药,以槐角、姜黄、川牛膝为代表的凉血止血、活血化瘀药,以枳实、车前子、灵芝、野山药为代表的补气理气药.该研究利用生物信息学分析预测放射性肺损伤发生的作用机制及潜在治疗的中药,为进一步的科学研究与临床应用提供参考.To explore the pathogenesis of radiation-induced lung injury and predict potential targeted therapies using traditional Chinese medicine,bioinformatics methods were employed to analyze the differentially expressed genes between irradiated lung tissue and normal lung tissue.The GSE202586 dataset was selected from the GEO database,and differentially expressed genes were identified through GEO_(2)R analysis.Functional enrichment analysis of these genes was conducted using GO,and signaling pathways were analyzed using KEGG.Protein-protein interaction networks were constructed using the STRING database,and core genes were identified using Cytoscape.Coremine Medical was then used to predict traditional Chinese medicine compounds that target these core genes.The results yielded a total of 113 differentially expressed genes,with 88 genes upregulated and 25 genes downregulated.These genes were primarily involved in biological and cellular processes,immune processes,and the regulation of signals,as well as activities related to non-transmembrane protein tyrosine kinases and signal receptors.The enriched pathways were mainly related to immune system signaling.Protein-protein interaction analysis revealed eight core genes,including CD19,CD22,CD52,CD86,LCP2,PAX5,SPN,and TNFRSF13C.Using these eight core genes,a targeted screening of traditional Chinese medicine was conducted,resulting in the identification of three categories of herbs.These included herbs with heat-clearing and detoxifying properties represented by Radix Paeoniae Rubra,Fructus Solani,Herba Diffusae Bidentatae,and Herba Solani;herbs with blood-cooling and hemostatic,as well as blood-circulation promoting properties represented by Semen Sophorae,Rhizoma Curcumae Longae,and Radix Cyathulae;and herbs with qi-tonifying and qi-regulating properties represented by Fructus Aurantii Immaturus,Semen Plantaginis,Ganoderma Lucidum,and Wild Yam.This study utilized bioinformatics analysis to predict the mechanisms underlying radiation-induced lung injury and potential thera

关 键 词：放射性肺损伤 差异基因 核心靶点 靶向中药 

分 类 号：R285[医药卫生—中药学]