坚硬黄耆正丁醇部位的化学成分研究  

Chemical constituents of n-butanol extract of Astragalus rigidulus

作　　者：吴昊芬 周家林 李文艳[2] 钟国跃 蒋伟 任刚 WU Haofen;ZHOU Jialin;LI Wenyan;ZHONG Guoyue;JIANG Wei;REN Gang(Chinese Medicine Resources and Ethnic Medicine Research Center,Jiangxi University of Chinese Medicine,Nanchang 330004,China;School of Nursing,Jiangxi University of Chinese Medicine,Nanchang 330004,China)

机构地区：[1]江西中医药大学中药资源与民族药研究中心,南昌330004 [2]江西中医药大学护理学院,南昌330004

出　　处：《广西植物》2024年第6期1060-1069,共10页Guihaia

基　　金：国家重点研发计划资助项目(2019YFC1712300);江西中医药大学校级研究生创新专项(JZYC20S40);国家自然科学基金面上项目(81873086)。

摘　　要：为探究藏药萨嘎尔(坚硬黄耆)正丁醇部位的化学成分,该研究采用HP-20大孔吸附树脂、Sephadex LH-20、ODS柱层析及半制备高效液相(PHPLC)对坚硬黄耆乙醇提取物正丁醇部位进行分离纯化,并采用NMR和HR-ESI-MS等波谱方法对所分离化合物进行结构鉴定。结果表明:从坚硬黄耆正丁醇部位分离得到19个黄酮衍生物和1个倍半萜苷,其结构分别为7-O-methylorobol-4′-O-β-D-葡萄糖苷(1)、mildiside A(2)、柚皮素(3)、樱黄素4′-O-β-D-葡萄糖苷(4)、orobot(5)、山柰酚-3-O-β-D-(6′-乙酰)葡萄糖苷(6)、5,7-二羟基-4′-甲氧基异黄酮-2′-O-β-D-葡萄糖苷(7)、amarantholidoside IV(8)、山柰酚-3-O-α-L-鼠李糖(1→2)-β-D-葡萄糖苷(9)、山柰酚(10)、5,7,4′-三羟基异黄酮(11)、山柰酚-3-O-β-D-葡萄糖苷(12)、(S)-mucronulatol(13)、毛蕊异黄酮(14)、槲皮素(15)、红车轴草素-7-O-β-D-葡萄糖苷(16)、2′-羟基-3′,4′-二甲氧基异黄烷-7-O-β-D-葡萄糖苷(17)、山柰酚-3-O-芸香糖苷(18)、5,7,4′-三羟基-3′-甲氧基黄酮醇-3-O-芸香糖苷(19)、槲皮素-3-O-β-D-葡萄糖苷(20)。化合物1-9为首次在黄耆属中分离得到,其余化合物均为首次在坚硬黄耆中分离得到。该结果为坚硬黄耆的药效物质研究提供了基础数据,为未来合理开发利用该植物资源提供了理论依据。To study the chemical constituents of n-butanol extract from Astragalus rigidulus,HP-20 macroporous adsorption resin,Sephadex LH-20 gel,ODS gel column chromatography and semi-preparative high performance liquid chromatography were used to separate the chemical constituents.The structures of all isolates were identified by spectroscopic methods,including NMR and HR-ESI-MS.The results showed that twenty compounds including nineteen flavonoid derivatives and one sesquiterpene glycoside were isolated and purified from n-butanol extract of A.rigidulus.Their structures were identified as 7-O-methylorobol-4′-O-β-D-glucopyranoside(1),mildiside A(2),naringenin(3),purine 4′-O-β-D-glucoside(4),orobot(5),kaempferol-3-O-β-D-(6′-acetyl)glucopyranoside(6),5,7-dihydroxy-4′-methoxyisoflavone-2′-O-β-D-glucopyranoside(7),amarantholidoside IV(8),kaempferol-3-O-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside(9),kaempferol(10),5,7,4′-trihydroxyisoflavone(11),kaempferol-3-O-β-D-glucopyranoside(12),(S)-mucronulatol(13),calycosin(14),quercetin(15),pratensein-7-O-β-D-glucoside(16),2′-hydroxy-3′,4′-dimethoxyisoflavan-7-O-β-D-glucoside(17),kaempferol-3-O-rutinoside(18),5,7,4′-trihydroxy-3′-methoxyflavonol-3-O-rutinoside(19),quercetin-3-O-β-D-glucoside(20).It is the first report for the compounds 1-9 found in the genus Astragalus.The other compounds are isolated from the title plant for the first time.The results of this study provide basic data for the pharmacodynamic material study of A.rigidulus,and provide a theoretical reference for the rational development and utilization of the plant resources in the future.

关 键 词：坚硬黄耆 化学成分 分离纯化 结构鉴定 异黄酮 

分 类 号：Q946[生物学—植物学]