蛇足石杉COBRA基因家族的分子生物信息学及表达分析  

Molecular bioinformatics and expression analysis of the COBRA gene family in Huperzia serrata

作　　者：黄玉妹 滕建北 涂冬萍 梁柳观 HUANG Yumei;TENG Jianbei;TU Dongping;LIANG Liuguan(College of Pharmacy,Guangxi University of Traditional Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,南宁530200

出　　处：《广西植物》2024年第6期1105-1117,共13页Guihaia

基　　金：广西自然科学基金(2022JJA140897);广西中医药大学桂派中医药传承创新团队项目(2022B005);广西中医药大学桂派杏林青年英才项目(2022C033);广西中医药大学研究生教育创新计划项目(YCSY2022007)。

摘　　要：为探明蛇足石杉COBRA基因家族成员分子生物信息学特征及组织表达规律,该文基于蛇足石杉的全长转录组数据,通过生物信息学技术对该家族成员(HsCOBRAs)的理化性质、结构域、保守基序、顺式作用元件、基因表达量等进行分析。结果表明:(1)在蛇足石杉全长转录组中共筛选出24个HsCOBRAs家族成员,其中酸性蛋白9个,稳定蛋白11个,疏水性蛋白5个,具有跨膜结构的蛋白7个,具有信号肽的蛋白3个。(2)亚细胞定位在细胞壁、叶绿体、细胞核、细胞膜上。(3)结构分析发现HsCOBRAs有7种结构域和6种保守基序,部分成员具有高度保守的CCVS结构。(4)HsCOBRAs具有CAAT-box、TATA-box等45种顺式作用元件。(5)HsCOBRA2在叶、孢子、茎、芽胞中的表达量均最高。该研究结果可为HsCOBRAs的进一步研究及生物学功能验证等提供理论依据。In order to clarify the molecular bioinformatics characteristics and tissue expression patterns of the COBRA gene family members of Huperzia serrata,the physicochemical properties,domains,conserved motifs,cis-acting elements,and genes expression of the family members(HsCOBRAs)were analyzed by bioinformatics techniques,based on the full-length transcriptome data of the H.serrata.The results were as follows:(1)A total of 24 HsCOBRAs family members were screened in the full-length transcriptome of H.serrata,including 9 acidic proteins,11 stabilizing proteins,5 hydrophobic proteins,7 proteins with transmembrane structures,and 3 proteins with signal peptides.(2)Subcellular localization was found in the cell wall,chloroplast,nucleus,and cell membrane.(3)Structural analysis revealed that HsCOBRAs had 7 domains and 6 conserved motifs,and partial members had a highly conserved CCVS structure.(4)HsCOBRAs had 45 cis-acting elements such as CAAT-box and TATA-box.(5)HsCOBRA 2 had the highest expressions in leaves,spores,stems and gemma.The study results can provide theoretical basis for further research and biological function verification of HsCOBRAs.

关 键 词：蛇足石杉 COBRA 基因家族 生物信息学 全长转录组 表达分析 

分 类 号：Q943[生物学—植物学]