止咳枇杷颗粒中桑皮苷A和绿原酸含量测定方法  

Determination of Mulberroside A and Chlorogenic Acid in Zhike Pipa Granules

作　　者：江丽苹[1] 梁经伟 李楚源[1] 李林媛 匡艳辉[1] JIANG Liping;LIANG Jinwei;LI Chuyuan;LI Linyuan;KUANG Yanhui(Guangzhou Baiyunshan Hutchison Whampoa Chinese Medicine Co.,Ltd.,Guangzhou 510515,China)

机构地区：[1]广州白云山和记黄埔中药有限公司,广东广州510515

出　　处：《中国民族民间医药》2024年第12期51-54,共4页Chinese Journal of Ethnomedicine and Ethnopharmacy

摘　　要：目的:应用高效液相色谱法测定止咳枇杷颗粒中桑皮苷A和绿原酸含量。方法:色谱柱为C18色谱柱,以乙腈-0.3%磷酸(9∶91)为流动相,流速1 mL/min,柱温30℃,进样量10μL,检测波长326 nm。结果:桑皮苷A在16.12~806.0μg/mL(r=1.0000)范围内线性关系良好,平均加样回收率为101.65%,RSD为2.10%(n=6);绿原酸在4.122~206.1μg/mL(r=1.0000)范围内线性关系良好,平均加样回收率为99.81%,RSD为0.56%(n=6)。结论:该方法测定结果准确、灵敏、重复性好,可用于止咳枇杷颗粒中桑皮苷A和绿原酸的含量测定。Objective To determine the content of Mulberroside A and Chlorogenic acid in Zhike Pipa granules by High-performance liquid chromatography.Method The chromatographic column is C 18,with acetonitrile 0.3%phosphoric acid(9∶91)as the mobile phase,flow rate of 1 mL/min,column temperature of 30℃,and injection volume of 10μL.The detection wavelength is 326nm.Result The linear relationship of mulberry skin Mulberroside A was good within the range of 16.12-806.0μg/mL(r=1.0000),with an average recovery rate of 101.65%and an RSD of 2.10%(n=6);The linear relationship of Chlorogenic acid is good within the range of 4.122-206.1μg/mL(r=1.0000),the average sample adding recovery is 99.81%,and the RSD is 0.56%(n=6).Conclusion The method is accurate,sensitive,and has good repeatability in determining results,and can be used for the determination of Mulberroside A and Chlorogenic acid in Zhike Pipa granules.

关 键 词：止咳枇杷颗粒 桑皮苷A 绿原酸 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]