肺结核患者Th1/Th2细胞状态与BALF中微生物群及Notch2 mRNA、IL-27 mRNA表达的相关性  

Relationship of Th1/Th2 Cell Status in Patients with Pulmonary Tuberculosis with Microbial Communities and Expression of Notch2 mRNA and IL-27 mRNA in BALF

作　　者：姜飞飞 许超 黄金华 徐沈津 JIANG Feifei;XU Chao;HUANG Jinhua(Department of Laboratory Medicine,Chongming Branch of the Tenth People's Hospital of Shanghai,Shanghai 202157)

机构地区：[1]上海市第十人民医院崇明分院检验科,上海202157 [2]上海市崇明区传染病医院检验科,上海202150

出　　处：《医学临床研究》2024年第6期853-856,共4页Journal of Clinical Research

摘　　要：【目的】探讨肺结核患者Th1/Th2细胞状态与支气管肺泡灌洗液(BALF)中微生物群及Notch2、白细胞介素-27(IL-27)表达的相关性。【方法】776例肺结核患者,按病情进展情况分为活动组(n=370)和非活动组(n=406),另选352例非肺结核呼吸系统疾病患者作为对照组,检测患者BALF中微生物群水平,Notch2 mRNA、IL-27 mRNA表达水平和外周血Th1、Th2细胞比例,分析Th1/Th2与BALF中微生物群、Notch2 mRNA、IL-27 mRNA表达的相关性。【结果】活动组、非活动组外周血Th1、Th2细胞比例及Th1/Th2值均低于对照组,活动组Th1细胞比例及Th1/Th2值低于非活动组(P<0.05),但两组Th2细胞比例比较,差异无统计学意义(P>0.05);活动组、非活动组嗜血杆菌、奈瑟菌数量,IL-27 mRNA表达高于对照组,链球菌数量、Notch2 mRNA表达低于对照组(P<0.05),且活动组嗜血杆菌、奈瑟菌数量,IL-27 mRNA高于非活动组,链球菌数量、Notch2 mRNA表达低于非活动组(P<0.05)。Pearson相关性分析显示,肺结核患者Th1/Th2值与链球菌数量及Notch2 mRNA表达呈正相关(P<0.05),与嗜血杆菌、奈瑟菌数量、IL-27 mRNA表达呈负相关(P<0.05)。【结论】肺结核患者的Th1/Th2细胞比例偏低,且随病情发展进一步降低,该免疫失衡与患者BALF中嗜血杆菌、奈瑟菌、链球菌感染及Notch2 mRNA、IL-27 mRNA表达有关。【Objective】To investigate the correlation of the Th1/Th2 cell status in patients with pulmonary tuberculosis(TB)with the microbial communities and expression of Notch2 and interleukin-27(IL-27)in bronchoalveolar lavage fluid(BALF).【Methods】A total of 776 TB patients were divided into an active group(n=370)and a non-active group(n=406).Additionally,352 patients with non-TB respiratory diseases were selected as the control group.The microbial levels in the BALF,the mRNA expression levels of Notch2 mRNA and IL-27 mRNA from BALF,and the peripheral blood Th1/Th2 cell ratio were measured to analyze the correlations of Th1/Th2 with microbial communities and Notch2 and IL-27 mRNA expression in BALF.【Results】Both active and non-active groups had lower cell number in peripheral blood Th1 and Th2 cells,the ratio of Th1/Th2 was also lower if compared to the control group.The active group had a lower proportion of Th1 cells and Th1/Th2 ratio than the non-active group(P<0.05),but there were no statistically significant differences in Th2 cell proportions between the two groups(P>0.05).Both active and non-active groups had higher counts of Haemophilus,Neisseria and higher IL-27 mRNA expression compared to the control group,while Streptococcus counts and Notch2 mRNA expression were lower(P<0.05).Moreover,the active group had higher counts of Haemophilus and Neisseria and higher IL-27 mRNA expression,but lower Streptococcus counts and lower Notch2 mRNA expression than the non-active group(P<0.05).Pearson correlation analysis showed that the Th1/Th2 ratio in TB patients was positively correlated with Streptococcus counts and Notch2 mRNA expression(P<0.05),and negatively correlated with Haemophilus and Neisseria counts and IL-27 mRNA expression(P<0.05).【Conclusion】The Th1/Th2 cell ratio is low in TB patients and further decreases with disease progression.This immune imbalance is associated with infections by Haemophilus,Neisseria,and Streptococcus in the BALF and with Notch2 mRNA and IL-27 mRNA expression.

关 键 词：结核 肺 Th1细胞/血液 Th2细胞/血液 支气管肺泡灌洗液/微生物学 

分 类 号：R521[医药卫生—内科学]