低共熔溶剂法提取连翘总黄酮工艺研究  

Study on Extraction Process of Total Flavonoids from Forsythia suspensa by Deep Eutectic Solvents Method

作　　者：周伟 梁旭华 杨文婷 程敏 Zhou Wei;Liang Xuhua;Yang Wenting;Cheng Min(Shaanxi Xiangju Pharmaceutical Group Co.,Ltd.,Shangluo,Shaanxi 726000,China;College of Biomedicine and Food Engineering,Shangluo University,Shangluo,Shaanxi 726000,China;Shaanxi Qinling Industrial Technology Research Institute of Special Biological Resources,Shangluo,Shaanxi 726000,China)

机构地区：[1]陕西香菊药业集团有限公司,陕西商洛726000 [2]商洛学院生物医药与食品工程学院,陕西商洛726000 [3]陕西秦岭特色生物资源产业技术研究院,陕西商洛726000

出　　处：《广西林业科学》2024年第3期315-323,共9页Guangxi Forestry Science

基　　金：陕西省科技创新团队项目(2022TD-56);陕西省重点研发计划项目(2024NC-YBXM-263);陕西省教育厅服务地方专项科研计划项目(22JC031);陕西省中医药管理局秦创原中医药创新研发转化项目(2022-QCYZH-042)。

摘　　要：为探索连翘(Forsythia suspensa)总黄酮提取最佳工艺,采用低共熔溶剂(DESs)法提取连翘不同部位(叶、果和茎)总黄酮;以氯化胆碱为氢键受体,分别以尿素、乙二醇、丙三醇、柠檬酸、葡萄糖、1,2-丙二醇和山梨醇为氢键供体,配置不同DESs组合,测定不同组合的总黄酮提取率;以DESs摩尔比和含水量及液固比、提取时间和提取温度为单因素变量,以总黄酮提取率为评价指标,采用响应面法确定最佳提取工艺。结果表明,氯化胆碱/丙三醇组合的总黄酮提取率最高;连翘果中的总黄酮含量最高,叶中的总黄酮含量次之,茎中的总黄酮含量最低。采用响应面法优化得到的最佳总黄酮提取工艺为氯化胆碱/丙三醇摩尔比1∶2、DESs含水量21%、液固比35 mL/g、提取时间125 min和提取温度70℃。采用低共熔溶剂法提取连翘不同部位总黄酮具有可行性,可为连翘总黄酮提取和深度开发提供理论和试验依据。In order to explore optimum extraction process of total flavonoids from Forsythia suspensa,total flavonoids from different parts of F.suspensa(leaves,fruits and stems)were extracted by deep eutectic solvents(DESs)method.Different combinations of DESs were configured with choline chloride as hydrogen-bond acceptor and urea,ethylene glycol,glycerol,citric acid,glucose,1,2-propanediol and sorbitol as hydrogen-bond donors respectively,and extraction rates of total flavonoids by different combinations were determined.Response surface method was employed to confirm optimum extraction process by adjusting molar ratio of DESs,water content of DESs,liquid-solid ratio,extraction time and extraction temperature as single factor variables,and extraction rate of total flavonoids as evaluation index.Results showed that combination of choline chloride/glycerol had the highest extraction rate of total flavonoids.Content of total flavonoids in F.suspensa fruits was the highest,followed by F.suspensa leaves.Content of total flavonoids in F.suspensa stems was the lowest.The optimum extraction process of total flavonoids optimized by response surface method was molar ratio of choline chloride/glycerol 1∶2,water content of DESs 21%,liquid-solid ratio 35 mL/g,extraction time 125 min and extraction temperature 70℃.It was feasible to extract total flavonoids from different parts of F.suspensa by DESs method,which could provide theoretical and experimental supports for extraction and deep development of total flavonoids from F.suspensa.

关 键 词：总黄酮 低共熔溶剂 响应面法 提取工艺 连翘 

分 类 号：S567.19[农业科学—中草药栽培] R284.2[农业科学—作物学]