Fe-Co普鲁士蓝比色法检测过氧化氢和谷胱甘肽  

Detection of hydrogen peroxide and glutathione based on Fe-Co prussian blue colorimetric method

作　　者：叶佳佳 张武翔 倪建聪 胡世荣 YE Jiajia;ZHANG Wuxiang;NI Jiancong;HU Shirong(College of Chemistry,Chemical Engineering and Environment,Minnan Normal University,Zhangzhou,Fujian 363000,China;College of Environment and Chemical Engineering,Jiangsu University of Science and Technology,Zhenjiang,Jiangsu 212003,China)

机构地区：[1]闽南师范大学化学化工与环境学院,福建漳州363000 [2]江苏科技大学环境与化工学院,江苏镇江212003

出　　处：《闽南师范大学学报（自然科学版）》2024年第2期52-63,共12页Journal of Minnan Normal University：Natural Science

基　　金：福建省自然科学基金项目(2019J01745)。

摘　　要：采用一锅水热法合成的Fe-Co普鲁士蓝类似物(PBAs)作为类过氧化物酶,基于H_(2)O_(2)与3,3',5,5'-四甲基联苯胺(TMB)的显色反应和GSH对氧化TMB(ox-TMB)的还原反应,建立一种用于H_(2)O_(2)和GSH定量分析的比色传感器.在H_(2)O_(2)为2.0~50μmol/L和GSH为6.6~50.63μmol/L范围内均表现出良好的线性关系,检测限分别为0.36和0.32μmol/L.Fe-Co Prussian blue analogs(PBAs)synthesized by a one-pot hydrothermal method were used as the peroxidase-like enzyme.Based on the color reaction of H_(2)O_(2)with 3,3',5,5'-tetramethylbenzidine(TMB)and the reduction reaction of glutathione(GSH)with oxidized TMB(ox-TMB),a colorimetric sensor for the quantitative analysis of H_(2)O_(2)and GSH was developed.The colorimetric sensor showed a favorable linear relationship in the range of 2.0-50μmol/L for H_(2)O_(2)and 6.6-50.63μmol/L for GSH,and the detection limits were 0.36 and 0.32μmol/L,respectively.

关 键 词：比色传感器 Fe-Co类普鲁士蓝 谷胱甘肽 过氧化氢 一锅水热法 

分 类 号：O622[理学—有机化学]