麒麟竭果实化学成分及其抑制血小板活性成分的研究  

Platelet-inhibitory Chemical Constituents in the Fruits of Daemonorops draco Bl.

作　　者：裴钰洁 庞道然 邹秋玉 王勃开 罗扬淦 黄豪 朱枝祥 霍会霞 赵云芳 屠鹏飞 李军[1,2] PEI Yujie;PANG Daoran;ZOU Qiuyu;WANG Bokai;LUO Yanggan;HUANG Hao;ZHU Zhixiang;HUO Huixia;ZHAO Yunfang;TU Pengfei;LI Jun(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 100029,China;Modern Research Center for Traditional Chinese Medicine,Beijing Research Institute of Chinese Medicine,Beijing University of Chinese Medicine,Beijing 100029,China;State Key Laboratory of Natural and Biomimetic Drugs,School of Pharmaceutical Sciences,Peking University,Beijing 100191,China)

机构地区：[1]北京中医药大学中药学院,北京100029 [2]北京中医药大学/北京中医药研究院/中药现代研究中心,北京100029 [3]北京大学药学院/天然药物及仿生药物国家重点实验室,北京100191

出　　处：《世界中医药》2024年第10期1386-1391,共6页World Chinese Medicine

基　　金：国家自然科学基金重点项目(82030114);国家中医药管理局青年岐黄学者培养项目。

摘　　要：目的:研究麒麟竭Daemonorops draco Bl.果实乙醇提取物(血竭)的乙酸乙酯提取部位中化学成分及其抑制血小板活化活性。方法:采用硅胶、ODS、LH-20凝胶柱色谱和半制备高效液相色谱技术对麒麟竭果实乙醇提取物的乙酸乙酯部位的化学成分进行分离纯化,结合^(1)H-NMR、^(13)C-NMR、MS等波谱学数据进行结构鉴定,采用凝血酶诱导的血小板活化释放ATP模型对化学成分进行活性筛选。结果:从麒麟竭果实乙醇提取物的乙酸乙酯提取部位中分离得到11个化合物。分别鉴定为Ethyl 4,6-dihydroxy-2-methoxy-3-methylbenzoate(1)、6-羟基-2,4-二甲氧基-3-甲基苯乙醇(2)、4,6-二羟基-2-甲氧基-3-甲基苯甲醛(3)、对羟基苯甲酸乙酯(4)、4-羟基苯甲酸甲酯(5)、3,5-二氯水杨酸(6)、对羟基苯甲醛(7)、Pinocembrin(8)、Strobopinin(9)、7-羟基-5-甲氧基-6-甲基二氢黄酮(10)和Ozoroalide(11)。结论:化合物1为新化合物,化合物2和3为新天然产物。化合物3、7和9可以明显地抑制凝血酶诱导的血小板活化释放ATP。Objective:To investigate the chemical constituents in the ethyl acetate fraction of the fruit extract of Daemonorops draco Bl.and their platelet-inhibitory activities.Methods:The ethyl acetate fraction of the fruit extract of D.draco was separated and purified by column chromatography with silica gel,ODS,and Sephadex LH-20 and semi-preparative HPLC.The structures of the chemical constituents were identified based on the spectral data from ^(1)H-NMR,^(13)C-NMR,and MS.The ATP release from thrombin-activated platelets was employed to screen the active constituents.Results:Eleven compounds were obtained from the ethyl acetate fraction of the fruit extract of D.draco.They were identified as ethyl 4,6-dihydroxy-2-methoxy-3-methylbenzoate(1),6-hydroxy-2,4-dimethoxy-3-methyl phenylethanol(2),4,6-dihydroxy-2-methoxy-3-methyl benzaldehyde(3),ethyl p-hydroxy benzoate(4),methyl 4-hydroxy benzoate(5),3,5-dichlorosalicylic acid(6),p-hydroxybenzaldehyde(7),pinocembrin(8),strobopinin(9),7-hydroxy-5-methoxy-6-methylflavanone(10),and ozoroalide(11).Conclusion:Compound 1 was a new compound and compounds 2 and 3 were new natural products.Compounds 3,7,and 9 significantly inhibited the ATP release from thrombin-activated platelets.

关 键 词：棕榈科 黄藤属 麒麟竭 化学成分 小分子酚酸 二氢黄酮 抑制血小板活化 

分 类 号：R284.2[医药卫生—中药学]