何首乌-牛膝药对抗维甲酸诱导骨质疏松作用及活性成分筛选研究  

Heshouwu(Radix Polygoni Multiflori)-Niuxi(Achyranthis Bidentatae Radix)Anti-Vitaminic Acid-Induced Osteoporosis and Screening of Active Ingredients

作　　者：韩艺杰 袁开智 王冰[1] 张振秋[1] 孙艳涛[1] HAN Yijie;YUAN Kaizhi;WANG Bing;ZHANG Zhenqiu;SUN Yantao(Liaoning University of Traditional Chinese Medicine,Dalian 116600,Liaoning,China)

机构地区：[1]辽宁中医药大学,辽宁大连116600

出　　处：《中华中医药学刊》2024年第7期255-258,I0012,共5页Chinese Archives of Traditional Chinese Medicine

基　　金：辽宁省自然科学基金项目(2019-MS-224);辽宁中医药大学大学生创新创业训练计划项目(X202010162071)。

摘　　要：目的研究何首乌-牛膝药对的抗骨质疏松作用及筛选活性成分。方法采取液相色谱-质谱(HPLC-MS)联用法分析何首乌-牛膝药对指纹共有峰并进行成分确认;采用维甲酸复制大鼠骨质疏松模型及酶联免疫吸附(ELISA)方法,探讨何首乌-牛膝药对的抗骨质疏松作用;应用SPSS分析色谱结果与肝、肾湿重、骨干湿比数据和ELISA测定结果的相关性筛选何首乌-牛膝药对抗骨质疏松活性成分。结果HPLC-MS联用法分析结果共确定10个共有指纹峰成分,分别为:没食子酸、杯苋甾酮、β-蜕皮甾酮、25-R-牛膝甾酮、二苯乙烯苷、25-S-牛膝甾酮、大黄素、大黄素甲醚、齐墩果酸-3-O-β-D-葡萄糖醛酸苷、牛膝皂苷D。生物学指标测定结果表明何首乌-牛膝药对能使维甲酸所致骨质疏松大鼠血清骨碱性磷酸酶(BALP)和骨钙素(BGP-OCN)指标升高,抗酒石酸酸性磷酸酶5b(TRACP-5b)和血清I型胶原C端肽(CTX-1)降低;何首乌-牛膝药对组肝、肾指数明显下降,骨干湿比上升,体质量变化明显。结论何首乌-牛膝药对具有抗骨质疏松作用,筛选出作用最显著活性成分为没食子酸、β-蜕皮甾酮、大黄素和齐墩果酸-3-O-β-D-葡萄糖醛酸苷。Objective To study the anti-osteoporosis effect of Heshouwu(Radix Polygoni Multiflori)-Niuxi(Achyranthis Bidentatae Radix)and the screening of activity ingredients.Methods HPLC-MS was used to analyze the common fingerprint peaks of Heshouwu(Radix Polygoni Multiflori)-Niuxi(Achyranthis Bidentatae Radix)and confirm their components.The vitaminic acid was used to copy the osteoporosis model and ELISA method was used to explore the anti-osteoporosis effect of Heshouwu(Radix Polygoni Multiflori)-Niuxi(Achyranthis Bidentatae Radix).The correlation of chromatographic results with the wet weights of liver and kidney,the ration of dry weight and wet weight of the bones and ELISA measurement results was analyzed by SPSS for screening the active ingredients of Heshouwu(Radix Polygoni Multiflori)-Niuxi(Achyranthis Bidentatae Radix)anti-osteoporosis.Results A total of 10 common fingerprint peak components were identified by HPLC-MS analysis:gallic acid,cyasterone,β-ecdysterone,25-R-achyosterone,stilbene glucoside,25-S-achyosterone,emodino,physcion,oleanolic acid 3-O-β-D-glucuro-nopyranoside,achyranthoside D.The results of biological indicators showed that Heshouwu(Radix Polygoni Multiflori)-Niuxi(Achyranthis Bidentatae Radix)can increase the bone alkaline phosphatase(BALP)and bone gla protein(BGP-OCN)indicators,and reduced the levels of tartrate resistant acid phosphatase 5b(TRACP-5b)and C-terminal telopeptides of type I collagen(CTX-1)of the osteoporosis rats caused by osteic acid.There was significant decrease in liver and kidney indexes,increase in the ratio of dry weight and wet weight of the bones and significant weight change after Heshouwu(Radix Polygoni Multiflori)-Niuxi(Achyranthis Bidentatae Radix)treatment.Conclusion Heshouwu(Radix Polygoni Multiflori)-Niuxi(Achyranthis Bidentatae Radix)has anti-osteoporotic effect,and the most significant active ingredients were gallic acid,β-ecdysterone,emodin and oleanolic acid-3-O-β-D-glucuro-nopyranoside.

关 键 词：何首乌-牛膝药对 骨质疏松 维甲酸 ELISA 活性成分 

分 类 号：R285.5[医药卫生—中药学]