白酒酿造底锅水资源化生产小球藻生长因子  

Resourceful Production of Chlorella Growth Factor Using Distilled Spirit Stillage

作　　者：姜翰达 严鹿鸣 李蓉[2] 史靖琪 张永奎[2] 谢通慧[2] JIANG Handa;YAN Luming;LI Rong;SHI Jingqi;ZHANG Yongkui;XIE Tonghui(Technology Innovation Center for Comprehensive Utilization of Strategic Mineral Resources,Ministry of Natural Resources,Chengdu 610041,China;School of Chemical Engineering,Sichuan University,Chengdu 610065,China)

机构地区：[1]自然资源部战略性矿产综合利用工程技术创新中心,四川成都610041 [2]四川大学化学工程学院,四川成都610065

出　　处：《化学与生物工程》2024年第7期37-43,共7页Chemistry & Bioengineering

基　　金：自然资源部战略性矿产综合利用工程技术创新中心开放基金项目(CCUM-KY-2302);中国轻工业浓香型白酒固态发酵重点实验室开放基金项目(2021JJ003);四川大学大学生创新创业训练计划项目(C2023126151)。

摘　　要：酿酒工业废水产生量大、成分复杂、营养物质丰富,若直接排放会对环境造成严重污染。以白酒酿造底锅水为培养液,利用小球藻进行生物转化生产小球藻生长因子(CGF),分析了白酒酿造底锅水中COD、磷酸磷(PO_(4)-P)、总磷(TP)、氨基氮(NH_(3)-N)、总氮(TN)的浓度变化及小球藻生物质的积累情况,采用噻唑蓝(MTT)法评价了白酒酿造底锅水转化产物CGF的生理活性。结果表明,小球藻可直接处理未稀释的白酒酿造底锅水,7 d后COD、TP、TN的去除率分别为74.41%、17.43%、51.32%,小球藻生物质干重积累量为8726.66 mg·L^(-1);当转化产物CGF浓度为0.2 mg·mL^(-1)时,小鼠成纤维细胞L929的细胞存活率达到最高,为138.80%。说明小球藻可显著降解白酒酿造底锅水中的COD、磷和氮(P<0.05),且转化产物CGF具有显著的生理活性,在低浓度时可促进细胞增殖。The brewing industry generates a large amount of wastewater with complex composition and high nutrient content,which will cause serious pollution to the environment if discharged directly.By using distilled spirit stillage as culture solution,Chlorella growth factor(CGF) was produced by biotransformation of Chlorella.We analyzed the concentration changes of COD,phosphorus phosphate(PO_(4)-P),total phosphorus(TP),amino nitrogen(NH_(3)-N),and total nitrogen(TN) in distilled spirit stillage and the biomass accumulation of Chlorella.Moreover,we evaluated the physiological activity of CGF from distilled spirit stillage by Methyl Thiazolyl Tetrazolium(MTT) colorimetry assay.The results show that Chlorella can directly treat the undiluted distilled spirit stillage,after 7 d,the removal rates of COD,TP,and TN are 74.41%,17.43%,and 51.32%,respectively,and the biomass dry weight accumulation of Chlorella is 8 726.66 mg·L^(-1).When the concentration of CGF is 0.2 mg·mL^(-1),the cell survival rate of mouse fibroblast L929 can reach the highest of 138.80%.The results indicate that Chlorella can significantly degrade COD,phosphorus,and nitrogen in distilled spirit stillage(P<0.05),the transformed product CGF has significant physiological activity,and the low concentration of CGF can promote cell proliferation.

关 键 词：白酒酿造底锅水 资源化利用 小球藻 小球藻生长因子 

分 类 号：TQ085[化学工程] X703[环境科学与工程—环境工程]