家蚕素斑品种菁松的茶斑限性定向转育  

The directional transfer of the plain silkworm variety Jingsong into a sex-limited multilunar silkworm variety

作　　者：廖鹏飞[1] 李琼艳[1] 罗顺高[1] 刘敏[1] 朱红涛[1] 李继娅[1] 白红英[1] 陈海佺[1] 范永慧[1] 董占鹏[1] LIAO Pengfei;LI Qiongyan;LUO Shungao;LIU Min;ZHU Hongtao;LI Jiya;BAI Hongying;CHEN Haiquan;FAN Yonghui;DONG Zhanpeng(Sericultural&Apicultural Research Institute,Yunnan Academy of Agricultural Sciences,Mengzi 661101,Yunnan,China)

机构地区：[1]云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101

出　　处：《浙江农业学报》2024年第5期1032-1040,共9页Acta Agriculturae Zhejiangensis

基　　金：向仲怀院士工作站(202305AF150166);云南省科技厅重点研发(农业领域)专项(202102AE090010);云南省现代农业蚕桑产业技术体系(2019KJTX006);现代农业产业技术体系建设专项(CARS-18)。

摘　　要：为在家蚕现行素斑品种菁松的幼虫发育早期进行雌雄性别鉴别,实现雌雄分养分缫差异化利用目的,用普斑限性品系云蚕7A的雌与茶斑品系Cb的雄杂交后,再分别与现行素斑品系菁松A、菁松B杂交,其后代连续数次回交素斑亲本后自交,结合分类选择进行个体和蛾区选择淘汰,将云蚕7A的普斑限性基因和Cb的茶斑基因转入现行素斑品种菁松中,定向转育得到菁A茶限和菁B茶限。结果表明:转育过程中,幼虫斑纹分化成颜色与形状易于分辨的茶斑与非茶斑2种类型,转育得到的菁A茶限和菁B茶限幼虫在3龄初期就会呈现茶斑颜色深浅明显不同的分化,能准确分辨雌雄,其虫蛹率分别为96.91%和96.40%,全茧量分别为1.62 g和1.56 g,茧层率分别为23.78%和23.26%,经济性状与其轮回亲本的差异较小,表现出健康好养、茧质优良等特性。研究结果可为创建特殊性状的育种材料、差异化利用雌雄蚕,以及降低雄蚕种繁育成本奠定基础。To identify the gender of the larvae in the early developmental stages of the current plain silkworm variety Jingsong,for the purpose of achieving sex-separation in rearing and differential utilization,the female of the sex-limited normal marking silkworm race Yuncan 7A was crossed with the male of multilunar silkworm race Cb.Then,these hybrids were respectively crossed with the commercial plain silkworm race Jingsong A and Jingsong B.The progeny were backcrossed to the commercial plain silkworm race for several times and then selfed,combined with categorized selection for individual and batches.This process introduced sex-limited normal marking gene of Yuncan 7A and multilunar gene of Cb into the commercial plain silkworm race Jingsong,directed breeding resulted in Jing A Chaxian and Jing B Chaxian.The results showed that during the breeding process,the larval spot differentiation turned into easy-to-distinguish multilunar and non-multilunar types by color and shape.The cultivated Jing A Chaxian and Jing B Chaxian larvae displayed clearly different color differentiation of multilunar at the beginning of the 3rd instar,allowing for accurate gender identification.The economic traits of Jing A Chaxian and Jing B Chaxian,such as the larva-pupa rate of 96.91%and 96.40%,the whole cocoon weight of 1.62 g and 1.56 g,the cocoon shell ratio of 23.78%and 23.26%,were slightly different from their recurrent parents,exhibiting characteristics of good health,easy rearing,and high-quality cocoons.This study laid a foundation for creating breeding materials with special traits,the differential utilization of male and female silkworms,and reducing the breeding costs of male silkworms.

关 键 词：家蚕品种 普斑限性 素斑 茶斑限性 定向转育 

分 类 号：S882[农业科学—特种经济动物饲养]