两株兔源多杀性巴氏杆菌分离鉴定及荚膜血清分型  

Identification and capsule serotyping of two strains of Pasteurella multocida isolated from rabbit

作　　者：季权安[1] 徐翔飞 鲍国连[1] 黄叶娥 崔雪梅 刘燕[1] JI Quan an;XU Xiangfei;BAO Guolian;HUANG Ye e;CUI Xuemei;LIU Yan(Institute of Animal Husbandry and Veterinary Science,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China)

机构地区：[1]浙江省农业科学院畜牧兽医研究所,浙江杭州310021

出　　处：《浙江农业学报》2024年第5期1041-1046,共6页Acta Agriculturae Zhejiangensis

基　　金：浙江省自然科学基金(LTGD24C180001);国家兔产业技术体系(CARS-43-C-2)。

摘　　要：为探明浙江省规模兔场主要传染病流行现状,明确其致病菌及血清型,开展了家兔流行病学调查与病原分离鉴定。调研发现,不少兔场存在以打喷嚏和鼻腔流出浆液性、黏液性或脓性分泌物为主要临床特征的呼吸道传染病,进而对浙江省家兔主产区发病严重兔场进行采样,从嵊州、宁波三家患病兔鼻腔中分离到两株病原菌,进行了病原分离鉴定、荚膜血清分型及药敏试验。根据分离菌的菌落形态、革兰氏染色特征、特异性PCR以及荚膜血清分型PCR鉴定结果,确定两株致病菌均为荚膜血清A型多杀性巴氏杆菌,分别命名为ZJNB0321和ZJSZ0322。构建系统发育树,结果表明,两分离株属于同一分支,且与多杀性巴氏杆菌处于同一分支,并拥有独立的分支。药敏试验结果表明,不同地区分离株药敏特性不尽相同。研究结果为兔场合理用药及有效防控提供了科学依据。To investigate the prevalence of major infectious diseases in rabbit farms in Zhejiang Province,the epidemiological survey was carried out to isolate,identify the serotype of pathogenic bacteria.It was found that respiratory infectious diseases,mainly characterized by sneezing and nasal discharge of serous,mucous or purulent secretions,existed in most rabbit farms.Thus,rabbits with severe respiratory diseases from two farms in Ningbo and Shengzhou were sampled for bacterial isolation and identification,serotyping,and drug sensitivity test.Based on the colonial morphology,characters of gram-staining,specific PCR,and capsule seroptyping,the bacterium were identified as capsule seroptype A Pasteurella multocida,which were named as ZJNB0321 and ZJSZ0322,respectively.The evolutionary tree results showed that the two isolates belonged to the same clade,and were in the same clade as Pasteurella multocida,but they had their own separate clade.The results of the drug sensitivity test indicated that the bacterium showed different drug sensitive characteristics.This study provides scientific basis for rational drug use,and development of effective control measures for rabbit farms.

关 键 词：兔 呼吸道疾病 多杀性巴氏杆菌 荚膜血清型 药敏试验 

分 类 号：S858[农业科学—临床兽医学]