两种树龄腾冲红花油茶花梗内生真菌的多样性  

Diversity analysis of endophytic fungi in the pedicels of two tree-age Camellia reticulate

作　　者：黄融 孟庆鑫 吴晓漫 荀利杰 张俊丽 董霞[2] 董坤 龚雪阳[1] HUANG Rong;MENG Qingxin;WU Xiaoman;XUN Lijie;ZHANG Junli;DONG Xia;DONG Kun;GONG Xueyang(Faculty of Animal Science and Technology,Yunnan Agricultural University/Eastern Bee Research Institute,Yunnan Agricultural University/Yunnan Provincial Engineering and Research Center for Sustainable Utilization of Honeybee Resources,Kunming 650201,China;College of Food Science and Technology,Yunnan Agricultural University,Kunming 650201,China)

机构地区：[1]云南农业大学动物科学技术学院/东方蜜蜂研究所/云南省蜜蜂资源可持续利用工程研究中心,云南昆明650201 [2]云南农业大学食品科技学院,云南昆明650201

出　　处：《浙江农业学报》2024年第5期1076-1085,共10页Acta Agriculturae Zhejiangensis

基　　金：国家自然科学基金项目(32060241);国家现代产业技术体系(蜜蜂)项目(CARS-44-KXJ13);云南省教育厅科学研究基金项目(2023Y0980);云南农业大学科研启动基金项目(KY2018-37)。

摘　　要：以腾冲红花油茶花梗为试材,采用高通量测序的方法,研究了12年生(N组)和60年生(O组)树龄的腾冲红花油茶花梗内生真菌群落结构及其多样性。结果表明,两种树龄腾冲红花油茶花梗内生真菌OTU和Alpha分析结果存在差异,物种多样性和丰富度差异显著。在两种树龄腾冲红花油茶花梗中子囊菌门和担子菌门是优势菌门(二者丰度之和>90%),但目、科水平上两组样品优势种群差异巨大,很多物种(真菌)只能分类到目水平,其中O组的煤炱目未分类科和N组银耳目未分类科占比最大。内生真菌功能预测得到11个生态共位群,未知(Unknown)占比最高,动物病原菌、植物病原菌、真菌寄生菌等与各种腐生菌组成了多功能的营养型,内生真菌中病原菌、寄生菌和腐生菌的丰度受树龄影响较大。综上所述,树龄大的腾冲红花油茶花梗中内生真菌多样性和丰富度显著增多,并且优势真菌种类、丰度和功能也发生改变,因此,利用内生真菌时树龄因素不可忽视。Taking the pedicel of Camellia reticulata as the test material,the community structure and diversity of endophytic fungi in the pedicel of Camellia reticulata at 12 and 60 years old were studied by high-throughput sequencing.The analysis results showed that there were differences in OTU and Alpha results of endophytic fungi in the pedicels of Camellia reticulata of two tree ages,and there were significant differences in species diversity and richness.Ascomycota and Basidiomycota were the dominant phyla(the sum of their abundance>90%)in the pedicels of Camellia reticulata of two tree ages,but the dominant populations of the two groups of samples at the level of order and family were very different.Many species(fungi)can only be classified to the level of order,among which unclassified_o_Capnodiales of the O group and unclassified_o_Tremellales of the N group accounted for a large proportion.The function of endophytic fungi was predicted to obtain 11 ecological symbionts,with the highest proportion of unknown.Animal pathogens,plant pathogens,fungal parasites,and various saprophytic bacteria formed a multifunctional nutrient type.The abundance of pathogens,parasites,and saprophytic bacteria in endophytic fungi was greatly affected by tree age.In summary,the diversity and richness of endophytic fungi in the pedicels of Camellia reticulata increased significantly,and the species,abundance,and function of dominant fungi also changed.Therefore,the age factor cannot be ignored when using endophytic fungi.

关 键 词：腾冲红花油茶 树龄 内生真菌多样性 高通量测序 

分 类 号：S794[农业科学—林木遗传育种]