温郁金根茎腐烂病的病原鉴定  

Pathogen identification of root rot of Curcuma wenyujin

作　　者：李亚妮 陈卫良[1] 毛碧增 LI Yani;CHEN Weiliang;MAO Bizeng(Institute of Biotechnology,Key Lab of Molecular Biology of Crop Pathogens and Insects,Ministry of Agriculture and Rural Affairs,Key Laboratory of Biology of Crop Pathogens and Insects of Zhejiang Province,Zhejiang University,Hangzhou 310058,China;The Rural Development Academy,Zhejiang University,Hangzhou 310058,China)

机构地区：[1]浙江大学生物技术研究所,农业农村部作物病虫分子生物学重点实验室,浙江省作物病虫生物学重点实验室,浙江杭州310058 [2]浙江大学新农村发展研究院,浙江杭州310058

出　　处：《浙江农业学报》2024年第5期1086-1093,共8页Acta Agriculturae Zhejiangensis

基　　金：农业农村部农业重大技术协同推广项目(2020XTTGZYC02);浙江省“三农六方”科技协作计划(CTZB-F160728AWZ-SNY1-37)。

摘　　要：在浙江省瑞安市陶山镇温郁金主产区,部分田块温郁金发生大规模倒伏、地下根茎严重腐烂。为明确引起该病害的病原物,利用LB培养基和TTC(2,3,5-氯化三苯基四氮唑)培养基从温郁金腐烂根茎中分离获得细菌菌株JF1,采用灌根法、芽侵染法和根茎侵染法确定该菌株对温郁金的致病性。通过形态学特征观察、生理生化测定、多基因序列(16S rDNA、rpoB、gyrB)系统发育分析,明确引起田间温郁金根茎腐烂的病原菌为桑肠杆菌(Enterobacter mori)。At main production areas of Curcuma wenyujin in Taoshan Town,Rui an City,Zhejiang Province,large-scale lodging of C.wenyujin and severe rot of their underground tubers occurred in some fields.To identify the pathogen that causes the disease,the bacteria strain JF1 was isolated from the rotten tuber of C.wenyujin by using LB and TTC(2,3,5-triphenyltetrazolium chloride)medium.The pathogenicity of isolated strain JF1 was confirmed by using three inoculation methods,including root irrigation,bud infection and rhizome infection.The taxonomic status of pathogenic bacteria was identified according to the morphological characteristics,physiological and biochemical tests,phylogenetic analysis of multiple gene sequences(16S rDNA,rpoB,gyrB).The results showed that the pathogenic bacteria causing root rot of C.wenyujin was Enterobacter mori.

关 键 词：温郁金 根茎腐烂 病原鉴定 桑肠杆菌 

分 类 号：S435[农业科学—农业昆虫与害虫防治]