南太湖水体抑藻菌的筛选分离及其活性成分鉴定  

Isolation of strains with algicidal activity from South Taihu Lake in China and identification of active compounds

作　　者：陈瑶瑶 刘雪萍 张绍勇 王继栋 王瑞俊 张立钦 CHEN Yaoyao;LIU Xueping;ZHANG Shaoyong;WANG Jidong;WANG Ruijun;ZHANG Liqin(Key Laboratory of Vector Biology and Pathogen Control of Zhejiang Province,College of Life Sciences,Huzhou University,Huzhou 313000,Zhejiang,China)

机构地区：[1]湖州师范学院生命科学学院,浙江省媒介生物学与病原控制重点实验室,浙江湖州313000

出　　处：《浙江农业学报》2024年第5期1124-1133,共10页Acta Agriculturae Zhejiangensis

基　　金：浙江省重点研发计划(2019C02030);湖州市自然科学基金(2020YZ06)。

摘　　要：采用稀释涂布法、改良分离培养基和16S rRNA基因序列对南太湖四季水体的可培养细菌进行分离、纯化与鉴定,筛选可抑制铜绿微囊藻(Microcystis aeruginosa)生长的活性细菌,并追踪其活性成分。结果表明:共分离得到420株可培养细菌,隶属于4门9纲20目35科66属192种,其中,黄杆菌属(Flavobacterium)、假单胞菌属(Pseudomonas)、短波单胞菌属(Brevundimonas)、芽孢杆菌属(Bacillus)的菌株数较多。16S rRNA基因序列结果显示,有20株细菌属于潜在新种。共分离到7株具有明显抑藻活性的细菌,其中,黄杆菌属菌株1-2-14的抑藻效果最佳,第7天时抑制率达71.52%。通过活性追踪,分离得到2个活性单体化合物cyclo(4-hydroxy-L-Pro-L-Leu)和pterodontriol B,其48 h抑藻活性分别为56.87%和67.81%。In the present study,culturable bacteria were isolated and purified from water samples of south Taihu Lake in four seasons by the dilution-plate method and modified isolation medium.These bacteria were identified using 16S rRNA gene sequence.Bacteria with algicidal activity against Microcystis aeruginosa were screened,and their active compounds were isolated and purified.It was shown that a total of 420 strains were isolated from water samples of south Taihu Lake in four seasons.They were classified into 192 species,66 genera,35 families,20 orders,9 classes and 4 phyla.Based on the isolated strain number,Flavobacterium,Pseudomonas,Brevundimonas and Bacillus were the majority at the genus level.There were 20 strains identified as potential new taxa by 16S rRNA gene sequence analysis.Seven strains showed obvious inhibitory activity against M.aeruginosa,of which Flavobacterium sp.1-2-14 exhibited the strongest algicidal activity,as its inhibition rate reached 71.52%after co-cultured for 7 days.By adopting the tracing method of bioactivity,two compounds,cyclo(4-hydroxy-L-Pro-L-Leu)and pterodontriol B were isolated,and their inhibition rates against M.aeruginosa were 56.87%and 67.81%at 48 h,respectively.

关 键 词：南太湖 可培养细菌 铜绿微囊藻(Microcystis aeruginosa) 抑藻活性 

分 类 号：Q939.9[生物学—微生物学] X524[环境科学与工程—环境工程]