珍稀濒危植物华顶杜鹃叶斑病病原菌的分离与鉴定  

Isolation and identification of the pathogen causing leaf spot disease on a rare and endangered plant species Rhododendron huadingense

作　　者：何佳薇 黄乐琴 卢振宇 方瑾 雷子阳 张慧娟[1] 蒋明[1] HE Jiawei;Huang Leqin;LU Zhenyu;FANG Jin;LEI Ziyang;ZHANG Huijuan;JIANG Ming(College of Life Sciences,Taizhou University,Taizhou 318000,Zhejiang,China)

机构地区：[1]台州学院生命科学学院,浙江台州318000

出　　处：《浙江农业学报》2024年第4期837-845,共9页Acta Agriculturae Zhejiangensis

基　　金：国家级大学生创新创业训练计划(202210350029)。

摘　　要：华顶杜鹃(Rhododendron huadingense)是浙江省特有的珍稀濒危植物,叶斑病是该植物的重要病害。本研究以华顶杜鹃叶斑病病叶为材料,在病原菌分离纯化、致病性鉴定的基础上,利用形态学和分子生物学手段确定病原菌的种类。结果表明,从病叶中分离到1株真菌,命名为HDDJ;柯赫氏法则验证结果表明,该菌能引起叶斑病,病斑特征与野外发病情况一致;HDDJ的菌落起初为白色,后变为灰白色,最后呈黑色,气生菌丝絮状,这些特征与葡萄座腔菌(Botryosphaeria dothidea)吻合;利用PCR方法克隆HDDJ肌动蛋白和微管蛋白基因片段,系统发育分析结果表明,它们均与葡萄座腔菌聚为一支。根据形态学与分子鉴定结果,确定引起华顶杜鹃叶斑病的病原菌为葡萄座腔菌。华顶杜鹃叶斑病病原菌的分离与鉴定为将来开展该病害的防治奠定了基础。Rhododendron huadingense is a rare and endangered plant species endemic to Zhejiang Province,and leaf spot is an important disease of this plant.R.huadingense leaves infected with leaf spot disease were used as materials.Based on pathogen isolation,purification,and pathogenicity identification,both morphological and molecular biology methods were applied to determine the fungal pathogen.The results showed that one fungal strain designated HDDJ was isolated from the diseased leaves.Using Koch s postulates,the fungus caused leaf spot disease,and the characteristics of the lesions were consistent with those observed in the field.The colonies of HDDJ were white,then gradually turned gray,and finally became black with fluffy aerial mycelium.The hyphal characteristics of HDDJ were identical to those of Botryosphaeria dothidea.Fragments of actin and tubulin genes were cloned by using PCR,and phylogenetic analysis results revealed that both were clustered with B.dothidea.According to the morphological and molecular identification results,it was confirmed that the fungal pathogen causing R.huadingense leaf spot disease was B.dothidea.Isolation and identification of R.huadingense leaf spot disease laid a foundation for this disease control in the future.

关 键 词：华顶杜鹃 叶斑病 葡萄座腔菌 分子鉴定 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治] S763.15[农业科学—植物保护]