云南省2016—2022年志贺氏菌分子分型及耐药性分析  

Investigation of Molecular Typing and Drug Resistance of Shigella in Yunnan Province from 2016 to 2022

作　　者：尹艳珠 杨祖顺[2] 邹颜秋硕 任翔 国译丹[2] 范璐[2] YIN Yanzhu;YANG Zushun;ZOU Yanqiushuo;REN Xiang;GUO Yidan;FAN Lu(Yangbi Center for Disease Control and Prevention,Yangbi Yunnan 672500;Inspection Center,Yunnan Center for Disease Control and Prevention,Kunming Yunnan 650022,China)

机构地区：[1]漾濞县疾病预防控制中心检验科,云南漾濞672500 [2]云南省疾病预防控制中心检验中心,云南昆明650022

出　　处：《昆明医科大学学报》2024年第7期79-86,共8页Journal of Kunming Medical University

基　　金：云南省重点科技专项计划基金资助项目(202002AE32005)。

摘　　要：目的对云南省2016—2022年食源性疾病主动监测分离的志贺氏菌进行血清、分子分型和药敏试验等分析,了解云南省志贺氏菌的病原学特性,为志贺氏菌感染的防控和治疗提供数据支持。方法2016年1月至2022年12月从患者粪便中分离志贺氏菌,采用玻片凝集法进行血清学分型,脉冲场凝胶电泳(PFGE)进行分子分型分析,微量肉汤稀释法进行药敏试验。结果共分离志贺氏菌89株,其中福氏志贺氏菌占52.81%,宋内志贺氏菌占47.19%。PFGE分析发现,2种志贺氏菌均分为A和B 2个聚类簇,B簇为优势簇。47株福氏志贺氏菌分为30种PFGE带型,有3组优势带型,42株宋内志贺氏菌分为22种PFGE带型,有4组优势带型,并识别两起疑似暴发事件。志贺氏菌对13种抗生素存在不同程度的耐药,78.65%的菌株为多重耐药菌株。结论云南省志贺氏菌PFGE优势带型明显,部分带型聚集分布。耐药形势严峻,多重耐药现象严重。福氏志贺氏菌和宋内志贺氏菌耐药表型存在差异。Objective To analyze the serum typing,PFGE molecular typing,and drug-resistance of Shigella strains isolated from the active surveillance for foodborne diseases in Yunnan province from 2016 to 2022,the aim is to understand the pathogen characteristics of Shigella in this period and provide the data support for the control and treatment of Shigella infection.Methods Shigella strains were isolated from the feces of patients between January 2016 and December 2022.Serological typing of the strains was performed using the slide agglutination.Pulsed field gel electrophoresis(PFGE)was employed to detect the molecular typing of the strains.Drug resistance test was achieved by the broth microdilution method.Results A total of 89 Shigella strains were isolated,of which Shigella flexneri accounted for 52.81%and Shigella sonnei 47.19%.Both Shigella flexneri and Shigella sonnei were divided into A and B clusters in PFGE analysis,with B as the predominant cluster.The PFGE bands of 47 Shigella flexneri strains were categorized as 30 types with 3 dominant classes,while 42 Shigella sonnei strains contained 22 types PFGE bands with 4 dominant classes.Two suspected outbreaks were distinguished.Shigella displayed varying degrees of drug resistance to 13 antibiotics,with a 78.65%of multiple resistant strains.Conclusion The dominant PFGE bands of Shigella in Yunnan province were clear,partial of which appeared the clustering distribution.The drug resistance of Shigella was severe and the phenomena of multiple drug resistance were very serious.Drug resistance prototypes between Shigella flexneri and Shigella sonnei were different.

关 键 词：志贺氏菌 血清型 脉冲场凝胶电泳 耐药性 

分 类 号：R378.2[医药卫生—病原生物学]