深低温保存下高效抗冻多肽的合理设计和机理探讨  

Rational Design of and Mechanism Insight into an Efficient Antifreeze Peptide for Cryopreservation

作　　者：Haishan Qi Yihang Gao Lin Zhang Zhongxin Cui Xiaojie Sui Jianfan Ma Jing Yang Zhiquan Shu Lei Zhang 

机构地区：[1]Department of Biochemical Engineering,School of Chemical Engineering and Technology,Frontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering(MOE),Tianjin University,Tianjin 300350,China [2]School of Engineering and Technology,University of Washington Tacoma,Tacoma,WA 98402,USA

出　　处：《Engineering》2024年第3期164-173,共10页工程（英文）

基　　金：supported by the National Key Research and Development Program of China (2021YFC2100800);the National Natural Science Foundation of China (22078238,21961132005,and 21908160);the Open Funding Project of the National Key Laboratory of Biochemical Engineering;the Program of Introducing Talents of Discipline to Universities (BP0618007)。

摘　　要：The development of effective antifreeze peptides to control ice growth has attracted a significant amount of attention yet still remains a great challenge.Here,we propose a novel design method based on in-depth investigation of repetitive motifs in various ice-binding proteins(IBPs)with evolution analysis.In this way,several peptides with notable antifreeze activity were developed.In particular,a designed antifreeze peptide named AVD exhibits ideal ice recrystallization inhibition(IRI),solubility,and biocompatibility,making it suitable for use as a cryoprotective agent(CPA).A mutation analysis and molecular dynamics(MD)simulations indicated that the Thr6 and Asn8 residues of the AVD peptide are fundamental to its ice-binding capacity,while the Ser18 residue can synergistically enhance their interaction with ice,revealing the antifreeze mechanism of AVD.Furthermore,to evaluate the cryoprotection potential of AVD,the peptide was successfully employed for the cryopreservation of various cells,which demonstrated significant post-freezing cell recovery.This work opens up a new avenue for designing antifreeze materials and provides peptide-based functional modules for synthetic biology.

关 键 词：Antifreeze peptides Evolution analysis Ice recrystallization inhibition Molecular dynamics simulation CRYOPRESERVATION Synthetic biology 

分 类 号：Q819[生物学—生物工程]