红景天苷促进人血管内皮细胞系EA.hy926增殖与迁移  

Salidroside promotes proliferation and migration of human vascular endothelial cell line EAhy926

作　　者：曹清文 祁琳 禹博 田晨晨 袁海宁 王越 CAO Qingwen;QI Lin;YU Bo;TIAN Chenchen;YUAN Haining;WANG Yue(College of Integrated Traditional Chinese and Western Medicine;Department of Pharmacy,Armed Police Characteristic Medical Center,Tianjin 300162;Department of Cardiology,Tianjin People′s Hospital,Tianjin 300122,China;College of Traditional Chinese Medicine,Tianjin University of Traditional Chinese Medicine,Tianjin 301617)

机构地区：[1]天津中医药大学中西医结合学院 [2]武警特色医学中心药剂科,天津300162 [3]天津市人民医院心内科,天津300122 [4]天津中医药大学中医学院,天津301617

出　　处：《基础医学与临床》2024年第7期925-930,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(81572852);天津市自然科学基金(18JCZDJC33200);天津中医药大学研究生创新基金(ZXYCXLX202119,ZXYCXLX202018,ZXYCXLX202213)。

摘　　要：目的探究红景天苷(SAL)对人血管内皮细胞系EA.hy926增殖及迁移的影响。方法实验分为对照组、1、10和100 nmol/L SAL组、10 nmol/L SAL+2μg/mL avastin[血管内皮生长因子(VEGF)阻断剂贝伐珠单抗]组、10 nmol/L SAL+2μg/mL IgG(阻断剂阴性对照)组、10 nmol/L SAL+8μg/mL avastin组、10 nmol/L SAL+8μg/mL IgG组、10μmol/L YC-1[低氧诱导因子-1α(HIF-1α)阻断剂]组和10μmol/L YC-1+10 nmol/L SAL组。MTS法和Transwell小室法检测EA.hy926细胞的增殖及迁移;RT-qPCR和Western blot检测HIF-1α和VEGF基因及蛋白水平;荧光素酶报告基因实验检测SAL对EA.hy926细胞HIF-1α转录活性的影响。然后,通过鸟苷酸环化酶激活剂(YC-1)作为HIF-1α阻断剂验证SAL能否通过HIF-1α影响VEGF的表达。结果SAL能显著促进EA.hy926细胞增殖(P<0.05),SAL(10 nmol/L)的促增殖作用被avastin(2μg/mL)显著减轻(P<0.05)。SAL能显著促进EA.hy926细胞的迁移(P<0.05),这一作用被avastin(8μg/mL)显著减轻(P<0.05)。SAL能够提高HIF-1α、VEGF基因和蛋白的表达水平并促进HIF-1α的转录活性(P<0.05),并且加入HIF-1α阻断剂YC-1后HIF-1α、VEGF蛋白水平下调(P<0.05)。结论HIF-1α/VEGF通路可能参与SAL促进EA.hy926细胞的增殖和迁移。Objective To investigate the effect of salidroside(SAL)on the proliferation and migration of human vascular endothelial cell line EA.hy926.Methods The cells were divided into control group and test groups of 1,10 and 100 nmol/L SAL,10 nmol/L SAL+2μg/mL avastin(vascular endothelial growth factor(VEGF)blocker)group,10 nmol/L SAL+2μg/mL IgG(blocker negative control)group,10 nmol/L SAL+8μg/mL avastin group,10 nmol/L SAL+8μg/mL IgG group,10μmol/L YC⁃1[hypoxia inducible factor⁃1α(HIF⁃1α)blocker]group and 10μmol/L YC⁃1+10 nmol/L SAL group.The proliferation and migration of EA.hy926 cells were detected by MTS assay and Transwell cell migration experiments.RT⁃qPCR and Western blot were used to measure the gene and protein level of HIF⁃1αand VEGF.The luciferase report gene experiment was used to find the effect of SAL on HIF⁃1αtranscription activity of EA.hy926 cells.The guanylate cyclase activator(YC⁃1)was used as a HIF⁃1αblocker to verify potential effect of SAL on the expression of VEGF through HIF⁃1α.Results SAL significantly promoted proliferation of EA.hy926 cells(P<0.05)and the proliferation promoting effect of SAL(10 nmol/L)was significantly reduced by the VEGF blocker bevacizumab avastin(2μg/mL)(P<0.05).SAL significantly promoted migration of EA.hy926 cells(P<0.05),and this effect was significantly inhibited by avastin(8μg/mL)(P<0.05).SAL increased the expression of HIF⁃1αand VEGF gene and protein,and promoted the transcription of HIF⁃1α(P<0.05).The level of HIF⁃1αand VEGF protein decreased by YC⁃1,a HIF⁃1αbloc⁃ker(P<0.05).Conclusions HIF⁃1α/VEGF pathway is potentially involved in SAL promoted proliferation and migration of EA.hy926 cells.

关 键 词：红景天苷 低氧诱导因子-1α(HIF-1α) 血管内皮生长因子(VEGF) 细胞增殖 细胞迁移 

分 类 号：R285[医药卫生—中药学]