重组人乳铁蛋白通过促进成骨细胞增殖加速大鼠骨折愈合  

Recombinant human lactoferrin accelerates fracture healing in rats by promoting osteoblast proliferation

作　　者：叶俊武 郑徐洲 费林聪 李漱阳 杨天府[3] YE Junwu;ZHENG Xuzhou;FEI Lincong;LI Shuyang;YANG Tianfu(Department of Bone and Joint Surgery,Affiliated Hospital of Southwest Medical University,Luzhou 646000;Sichuan Provincial Laboratory of Orthopaedic Engineering,Luzhou 646000;Department of Orthopedics,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]西南医科大学附属医院骨与关节外科,四川泸州646000 [2]四川省骨科置入器械研发及应用技术工程实验室,四川泸州646000 [3]四川大学华西医院骨科,四川成都610041

出　　处：《基础医学与临床》2024年第7期989-996,共8页Basic and Clinical Medicine

基　　金：西南医科大学附属医院博士启动基金(22028)。

摘　　要：目的研究重组人乳铁蛋白(rhLF)对模型大鼠股骨骨折愈合及大鼠原代成骨细胞增殖的影响。方法将雄性大鼠随机分为3组:单纯骨折组(SF组)、胶原膜处理组(CF组)和rhLF复合胶原膜处理组(CLF组)。通过X线胶片及HE和Masson染色组织学观察比较骨折愈合情况。培养的24 h新生SD大鼠原代成骨细胞以不同浓度rhLF进行处理,MTT法检测成骨细胞增殖。结果CLF组在术后1、2、3周时骨痂较SF组及CF组体积增加更明显(P<0.05),4周时组间对比差异无统计学意义。CLF组软骨痂和硬骨痂增殖更明显,并率先出现成熟骨小梁和板层骨,骨痂成熟度高。与对照组比较,第1天100 mg/L和500 mg/L rhLF组对体外培养的大鼠原代成骨细胞,吸光度(A)值有明显降低(P<0.05)。第5天及第10天时随rhLF浓度增加,A值明显升高(P<0.05)。结论在体内rhLF具有加速骨折愈合作用,表现为骨痂形成快、体积大、成熟度高。它也可以促进体外培养的成骨细胞增殖,主要表现在成骨细胞快速增殖阶段。Objective To investigate the effect of recombinant human lactoferrin(rhLF)on fracture healing in rat models with femur fracture and the proliferation of rat primary osteoblasts.Methods Male rats were randomly di⁃vided into 3 groups:simple fracture group(SF group),collagen film⁃treated group(CF group)and rhLF⁃com⁃pound collagen lamination film⁃treated group(CLF group).The fracture healing was observed by X⁃ray image and histological microscopy with HE and Masson staining.The newborn SD rats with osteoblasts were treated with differ⁃ent concentrations of rhLF,and osteoblast proliferation was detected by MTT method.Results The bone scabs in the CLF group were significantly larger than those in the SF and CF groups at 1,2,and 3 weeks postoperatively(P<0.05).There was no difference in the comparison between the groups at 4th week.The proliferation of cartilag⁃inous and sclerotic bone scabs in the CLF group was more pronounced and showed the appearance of mature trabec⁃ulae and lamellar bone with a high degree of maturation of the bone scabs.The groups with 100 mg/L and 500 mg/L rhLF showed a significant decrease in A value for rat primary osteoblasts cultured in vitro as compared with the control group(P<0.05).The A value increased significantly at the first day(P<0.05).The concentration of rhLF increased at the fifth and tenth days.Conclusions rhLF has an accelerating effect on fracture healing which is manifested by fast scab formation,large volume and high maturity.The rhLF can promote the proliferation of os⁃teoblasts cultured in vitro,and the effect is mainly manifested in the rapid proliferation stage of osteoblasts.

关 键 词：重组人乳铁蛋白 骨折愈合 成骨细胞 股骨骨折 大鼠 

分 类 号：R683[医药卫生—骨科学]