人羊膜间充质干细胞对大鼠子宫瘢痕的修复作用  

Repair effect of human amniotic mesenchymal stem cells on uterine scars in rats

作　　者：宋佳 赵峰[2] 张婷 徐静 孙静莉 陈震宇 SONG Jia;ZHAO Feng;ZHANG Ting;XU Jing;SUN Jingli;CHEN Zhenyu(Department of Obstetrics and Gynecology,General Hospital of Northern Theater Command,Shenyang 110016;Department of Stem Cells and Regenerative Medicine,China Medical University,Shenyang 110122;Department of Obstetrics and Gynecology,Jinzhou Medical University,Jinzhou 121001,China)

机构地区：[1]北部战区总医院妇产科,辽宁沈阳110016 [2]中国医科大学干细胞与再生医学研究室,辽宁沈阳110122 [3]锦州医科大学妇产科学系,辽宁锦州121001

出　　处：《基础医学与临床》2024年第7期1002-1007,共6页Basic and Clinical Medicine

基　　金：辽宁省科学技术计划(2022JH2,101500029)。

摘　　要：目的探究人羊膜间充质干细胞(hAMSCs)对大鼠子宫瘢痕的修复作用。方法分离培养hAMSCs,选雌性SPF级SD大鼠行子宫壁全层切开术,术中注射hAMSCs,于术后第30天对子宫切开部位进行组织学检查。用ImageJ图像分析软件进行分析比较各组子宫肌层厚度、纤维化面积百分比。免疫组化法分别检测子宫肌层α平滑肌肌动蛋白(α-SMA)、转化生长因子β1(TGF-β1)、肿瘤增殖抗原Ki-67(Ki-67)阳性面积百分比。结果与磷酸缓冲盐溶液(PBS)组相比,hAMSCs组子宫肌层明显增厚、纤维化面积减少,α-SMA、Ki-67阳性表达明显增加(P<0.05),而TGF-β1阳性表达明显减少(P<0.05)。。结论人羊膜间充质干细胞(hAMSCs)可能通过减少瘢痕纤维化的形成、促进子宫瘢痕处平滑肌细胞的增殖等作用机制促进子宫切口组织修复。Objective To exploring the effect of human amniotic mesenchymal stem cells(hAMSCs)on the re⁃pair of rat uterine scars.Methods The hAMSCs were isolated and cultured,Female SPF grade SD rats were selected for full⁃thickness incision of uterine wall and then implanted with hAMSCs.On the 30th day after op⁃eration,the uterine incision was examined histologically.ImageJ image analysis software was used to analyze and to compare the thickness of uterine myometrium and the percentage of fibrotic area in each group.Immuno⁃histochemical method was used for detecting the percentage of positive areas ofα⁃SMA,TGF⁃β1,and Ki⁃67.Results Compared with the PBS group,the hAMSCs group showed significant thickening of the uterine mus⁃cle layer and fibrotic area was decreased,The positive expression ofα⁃SMA,and Ki⁃67 significantly increased(P<005),while the expression of TGF⁃β1 was significantly reduced(P<005).Conclusions The hAMSCs may promote the repair of uterine incision scars by reducing the formation of scar fibrosis and promoting the proliferation of smooth muscle cells in uterine scars.

关 键 词：人羊膜间充质干细胞 剖宫产 子宫瘢痕 

分 类 号：R719.8[医药卫生—妇产科学]