基于网络药理学和实验验证探讨海蓬子抗氧化和抗炎的作用机制  

Exploring the mechanism of antioxidant and anti-inflammatory effect of Salicornia europaea L. based on network pharmacology and experimental validation

作　　者：毕凤荷 王冉冉[1] 乔卫[1] BI Fenghe;WANG Ranran;QIAO Wei(Tianjin Key Laboratory on Technologies Enabling Development of Clinical Therapeutics and Diagnostics,Biopharmacy,College of Pharmacy,Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]天津市临床药物关键技术重点实验室,天津医科大学药学院生药学,天津300070

出　　处：《天津医科大学学报》2024年第4期332-337,共6页Journal of Tianjin Medical University

摘　　要：目的:通过网络药理学结合实验验证海蓬子抗氧化、抗炎的作用机制。方法:使用PubMed、TCMID、CNKI等数据库,对海蓬子关键活性成分及其相关目标靶点进行筛选。利用疾病数据库GeneCards、OMIM搜索与抗氧化、抗炎相关的疾病靶点。通过在线软件Venn2.1.0得到潜在靶点,绘制韦恩图,然后利用Cytoscape3.9.1、STRING技术平台,建立“疾病-植物-成分-靶点-信号通路”和蛋白-蛋白互作网络图,将靶点输入David6.8数据库进行富集分析,并通过检测谷胱甘肽(GSH)、丙二醛(MDA)等含量指标进行海蓬子抗氧化作用的动物实验验证。结果:研究获得20个海蓬子活性成分,与这些成分相关对应靶点308个;与抗氧化、抗炎相关靶点分别为1222、1868个,海蓬子与抗氧化、抗炎的交集靶点为207个。对PPI网络拓扑分析,得出5-羟色胺受体1A(HTR1A)、多巴胺受体D2(DRD2)、钠依赖性多巴胺转运体(SLC6A3)等多个抗氧化和抗炎的潜在靶点基因。GO功能富集分析总共得到989个候选基因,通过对KEGG信号通路富集分析显示主要与磷脂酰肌醇3激酶(PI3K)/蛋白激酶B(Akt)信号通路、脂质和动脉粥样硬化及蛋白聚糖有关。海蓬子提取物各剂量组体重显著降低(F=3.85,P<0.01);与模型组比较,各给药组的MDA含量显著降低(F=7.84,P<0.001),GSH含量显著升高(Z=-5.24,P<0.001)。结论:海蓬子可以通过多个靶点、多个成分发挥抗氧化、抗炎症的作用。Objective:To explore the mechanism of antioxidant and anti-inflammatory effects of Salicornia europaea L.through network pharmacology combined with experimental validation.Methods:Screening of key active ingredients and their related targets of Salicornia europaea L.was carried out using PubMed,TCMID and CNKI databases.Two disease databases,GeneCards and OMIM,were utilized to search for disease targets related to antioxidant and anti-inflammatory.The potential targets were obtained through the online software Venn2.1.0,the Wayne diagram was drew,and then Cytoscape3.9.1 and STRING technology platforms were utilized to establish the"Disease-Plant-Component-Target-Signaling Pathway"and protein-protein interactions network diagrams.The target points were entered into the David6.8 database for enrichment analysis,and animal experimental verification of the antioxidant effect of Salicornia europaea L.was carried out by testing glutathione(GSH),malondialdehyde(MDA)and other content indicators.Results:Twenty active components and 308 targets related to these components were obtained from Salicornia europaea L.The number of targets related to antioxidant and anti-inflammatory were 1222 and 1868,respectively,and the number of targets at the intersection between Salicornia europaea L.and antioxidant and anti-inflammatory effects was 207.PPI network topology analysis showed that serotonin receptor 1A(HTR1A),dopamine receptor D2(DRD2)and sodium dependent dopamine transporter(SLC6A3)were potential target genes for antioxidant and anti-inflammatory activities.GO functional enrichment analysis yielded a total of 989 candidate genes,which were mainly related to the PI3K/Akt signaling pathway,lipids,atherosclerosis and proteoglycans,as shown by the enrichment analysis of KEGG signaling pathway.The body weight of each dose group of Salicornia europaea L.extract significantly decreased(F=3.85,P<0.01).Compared with the model group,the MDA content in each treatment group was significantly reduced(F=7.84,P<0.001),while the GSH content

关 键 词：海蓬子 网络药理学 抗氧化 抗炎症 

分 类 号：R285.5[医药卫生—中药学]