春尺蠖幼虫生防细菌的分离鉴定及杀虫活性测定  

Isolation,Identification,and Insecticidal Activity Determination of Biocontrol Bacteria from the Larvae of Apocheima cinerarius

作　　者：王海旭 张婷婷[1] 王新谱[1] Wang Haixu;Zhang Tingting;Wang Xinpu(School of Agriculture,Ningxia University,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院,宁夏银川750021

出　　处：《农业科学研究》2024年第2期33-38,57,共7页Journal of Agricultural Sciences

基　　金：宁夏重点研发计划项目(2021BEG02009)。

摘　　要：为筛选出对鳞翅目幼虫有致病作用的昆虫病原菌,从春尺蠖(Apocheima cinerarius)幼虫的罹病体中分离获得疑似致病细菌4株,依据科赫氏法则最终筛选出1株具有杀虫活性的菌株CH-1。基于生理生化及16S rRNA序列鉴定,菌株CH-1为短小芽孢杆菌(Bacillus pumilus)。以小菜蛾(Plutella xylostella)、甜菜夜蛾(Spodoptera exigua)、草地贪夜蛾(Spodoptera frugiperda)、春尺蠖(Apocheima cinerarius)幼虫进行短小芽孢杆菌CH-1杀虫活性的测定。结果表明:短小芽孢杆菌CH-1发酵液及上清液均对小菜蛾等鳞翅目幼虫具有杀虫活性和致死效果,其中发酵液效果较上清液更为显著。在处理4 d后,发酵液处理下小菜蛾、甜菜夜蛾、草地贪夜蛾、春尺蠖的3龄幼虫死亡率分别为96.67%、100%、100%、100%,比清水对照的死亡率分别高40.00%、70.00%、40.00%、40.00%,比上清液处理的死亡率分别高0、13.33%、60.00%、0。试验得出,短小芽孢杆菌CH-1具有防治多种鳞翅目害虫的应用潜力。In order to screen out the insect pathogens with pathogenic effects against lepidoptera larvae,four suspected pathogenic bacteria were isolated from diseased Apocheima cinerarius larvae,and one strain CH-1 was ultimately screened out for its insecticidal activity using Koch’s postulates.The strain CH-1 was identified as Bacillus pumilus based on physiologicalbiochemical characteristics and 16S rRNA sequence analysis.Larvae of Plutella xylostella,Spodoptera exigua,Spodoptera frugiperda and A.cinerarius were then used to determine the insecticidal activity of B.pumilus CH-1.The results showed that both the fermentation broth and supernatant of B.pumilus CH-1 exhibited insecticidal and lethal effects on lepidoptera larvae such as P.xylostella,with the fermentation broth being more effective.After four days of treatment with the fermentation broth,the mortality rates of the third instar larvae of P.xylostella,S.exigua,S.frugiperda and A.cinerarius were 96.67%,100.00%,100.00%,and 100.00%,respectively,which were 40.00%,70.00%,40.00%,and 40%higher than those of the clean water control,and 0%,13.33%,60.00%,and 0%higher than those treated with the supernatant,respectively.The study indicates that Bacillus pumilus CH-1 has potential applications in the control of various lepidopteran pests.

关 键 词：春尺蠖 短小芽孢杆菌 分离鉴定 杀虫活性 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]