防风对春季低温胁迫的生理响应  

Physiological Response of Saposhnikovia divaricate to Cold Stress in Spring

作　　者：刘文斐 张炜 王力扬 姚瑶 宋孝文 孟祥才[1] LIU Wen-fei;ZHANG Wei;WANG Li-yang;YAO Yao;SONG Xiao-wen;MENG Xiang-cai(Heilongjiang University of Chinese Medicine,Harbin 150040,China)

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《中国现代中药》2024年第6期1036-1047,共12页Modern Chinese Medicine

基　　金：国家自然科学基金区域联合项目(U20A20407);黑龙江省中医药管理局道地药材标准(团体)制定项目(ZHYCYC2022-003)。

摘　　要：目的:探究防风对春季低温胁迫的生理响应,根据次生代谢产物含量确定防风的最佳采收时间。方法:在秋末和春季分别采收防风鲜根,比较防风内活性氧含量、抗氧化酶活性、次生代谢产物关键酶活性、次生代谢产物含量和总多糖、酸性多糖含量之间的关系。结果:在春季低温的影响下,防风鲜根中活性氧和丙二醛的含量,超氧化物歧化酶、过氧化氢酶和过氧化物酶活性,防风次生代谢产物关键酶苯丙氨酸裂解酶、乙酰辅酶A羧化酶、查耳酮合成酶活性及代谢途径产物1,3-二磷酸甘油酸含量比秋季均有显著提高。与秋季比较,春季防风中升麻素苷、5-O-甲基维斯阿米醇苷和升麻素含量分别提高了17.7%、36.7%、11.1%;秋季防风总多糖和酸性多糖含量均高于春季。结论:防风抗氧化酶和次生代谢产物共同作用维持机体在春季低温胁迫下活性氧的平衡,春季采收可显著提高色原酮含量。Objective:To investigate the physiological response of Saposhnikovia divaricate to cold stress in spring and determine the best harvest time of S.divaricate according to the content of secondary metabolites.Methods:The fresh roots of S.divaricate were harvested at the end of autumn and in spring,respectively,and the relationships between the reactive oxygen species(ROS)content,antioxidant enzyme activities,key enzyme activity of secondary metabolites,secondary metabolite content,total polysaccharide content,and acid polysaccharide content were investigated.Results:Affected by the low temperatures in spring,the content of ROS and malondialdehyde(MDA),the activities of superoxide dismutase(SOD),catalase(CAT),and peroxidase(POD),the activities of key enzymes of secondary metabolites including phenylalanine ammonia-lyase(PAL),acetyl CoA carboxylase(ACC),and chalcone synthase(CHS),and the content of 1,3-disphosphoglycerate(1,3-DPG),a product of the metabolic pathway in the fresh roots of S.divaricate were significantly increased compared with those in autumn.Compared with that in autumn,the content of prim-O-glucosylcimifugin,5-Omethylvisamminol,and cimifugin in spring was increased by 17.7%,36.7%,and 11.1%,respectively,while the content of total polysaccharide and acid polysaccharide in autumn was higher than that in spring.Conclusion:The antioxidant enzymes and secondary metabolites of S.divaricate synergistically maintain the balance of ROS under cold stress in spring,and spring harvest can increase the content of chromone.

关 键 词：防风 采收期 活性氧 次生代谢 色原酮 

分 类 号：R282.2[医药卫生—中药学]