黄瓜FRK基因家族的鉴定及其表达分析  

Identification and Expression Analysis of FRK Gene Family in Cucumber(Cucumis sativus)

作　　者：马娟娟 侯栋 黄书超[1] 张东琴 岳宏忠[2] 李亚莉[2] 颉建明[1] MAJuan-Juan;HOU Dong;HUANG Shu-Chao;ZHANG Dong-Qin;YUE Hong-Zhong;LI Ya-Li;XIE Jian-Ming(College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China;Vegetable Research Institute,Gansu Academy of Agricultural Sciences,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学园艺学院,兰州730070 [2]甘肃省农业科学院蔬菜研究所,兰州730070

出　　处：《农业生物技术学报》2024年第7期1483-1493,共11页Journal of Agricultural Biotechnology

基　　金：国家产业技术体系兰州综合试验站项目(CARS-23-G-19)。

摘　　要：果糖激酶(fructokinase,FRK)是果糖磷酸化过程中主要的催化酶,在调节植物生长发育过程中起关键作用。为了深入研究黄瓜(Cucumis sativus)FRK基因功能,本研究对黄瓜FRK基因家族及其分子特征进行系统鉴定和分析,并对非生物胁迫下的基因表达模式进行分析,结果显示,黄瓜FRK基因家族有12个成员,分布于7条染色体上;编码氨基酸个数为331~588,分子量为35.63~65.90 kD;系统进化分析将黄瓜FRK基因家族划分为3个亚族,每个亚族中的基因结构和保守基序基本一致;启动子序列主要含有光响应、低温响应、干旱响应、激素响应、防御应激和植物生长发育相关等顺式作用元件。组织特异性表达分析显示,黄瓜部分FRK基因在根、茎、叶、雌花和雄花等组织器官中的表达量均较低或不表达,且黄瓜FRK基因具有组织特异性;非生物胁迫表达分析显示,CsaFRK6、CsaFRK8和CsaFRK12在低温胁迫下显著上调表达(P<0.05);CsaFRK2、CsaFRK5和CsaFRK7在盐胁迫下显著上调表达(P<0.05);CsaFRK1~CsaFRK9在干旱胁迫下显著上调表达(P<0.05),表明黄瓜FRK基因在不同组织器官以及响应非生物胁迫时的表达模式存在差异。本研究为阐明黄瓜的基因功能及响应非生物胁迫提供了参考。Fructokinase(FRK)is the main catalytic enzyme in the phosphorylation of fructose and plays a key role in the regulation of plant growth and development.In order to further study the FRK gene function of Cucumis sativus,the FRK gene family and its molecular characteristics were systematically identified and analyzed,and the gene expression patterns under abiotic stress were analyzed.The results showed that there were 12 FRK genes distributed on 7 chromosomes.The number of encoded amino acids was 331~588,and the molecular weight was 35.63~65.90 kD.The FRK gene family of cucumber was divided into 3 subfamilies by phylogenetic analysis,and the gene structure and motif in each subfamily were basically the same.The promoter sequences mainly contained cis-acting elements such as light response,low temperature response,drought response,hormone response,defense stress and plant growth and development.Tissue-specific expression analysis showed that the expression levels of some FRK genes in cucumber were low or not expressed in roots,stems,leaves,female flowers,and male flowers,indicated that different cucumber FRK genes had tissue-specific expression patterns.Abiotic stress expression analysis showed that CsaFRK6,CsaFRK8 and CsaFRK12 was significantly up-regulated under low temperature stress(P<0.05).The expression of CsaFRK2,CsaFRK5 and CsaFRK7 was significantly up-regulated under salt stress(P<0.05).CsaFRK1~CsaFRK9 were significantly up-regulated under drought stress(P<0.05).The expression patterns of FRK genes in different tissues and organs were different in response to abiotic stress.This study provides a reference for elucidating the biological gene function of cucumber and its response to abiotic stress.

关 键 词：黄瓜 果糖激酶(FRK) 非生物胁迫 

分 类 号：S642.2[农业科学—蔬菜学]