绵羊PRSS12基因多态性及其与生长性状的关联分析  

Polymorphism of PRSS12 Gene and Its Association Analysis with Growth Traits in Sheep(Ovis aries)

作　　者：熊金珂 鲍晶晶 毕亚珍 胡文萍[1] 张莉[1] XIONG Jin-Ke;BAO Jing-Jing;BI Ya-Zhen;HUWen-Ping;ZHANG Li(Institute of Animal Science,Chinese Academy of Agricultural Sciences/State Key Laboratory of Animal Biotech Breeding,Beijing 100193,China)

机构地区：[1]中国农业科学院北京畜牧兽医研究所/畜禽生物育种全国重点实验室,北京100193

出　　处：《农业生物技术学报》2024年第7期1587-1595,共9页Journal of Agricultural Biotechnology

基　　金：内蒙古自治区揭榜挂帅(2022JBGS0024);新疆天池英才计划人才项目(新疆畜牧科学院);国家重点研发计划(2021YFD1300902)

摘　　要：绵羊(Ovis aries)生长性状相关分子标记的鉴定对于提高绵羊产业的经济效益具有重要意义。丝氨酸蛋白酶12(serine protease 12,PRSS12),又称为神经胰蛋白酶,在中枢神经系统特异性表达,与多种神经功能相关。为探究PRSS12基因的多态性及其与湖羊和乌珠穆沁羊生长性状之间的相关性,本研究采用基因芯片技术对1008只湖羊和336只乌珠穆沁羊进行基因分型,并与生长性状进行关联分析,以鉴定绵羊生长发育相关分子标记。基因分型结果表明,在乌珠穆沁羊群体中检测到2个SNP位点,其中rs402399470G>A位点呈现低度多态,rs418440631G>A位点呈现中度多态;在湖羊群体中仅检测到1个SNP位点,即rs418440631G>A,表现为低度多态。使用一般线性模型将乌珠穆沁羊和湖羊的基因型和生长性状数据进行相关性分析,结果表明,rs402399470G>A位点与乌珠穆沁羊4月龄体重和6月龄胸宽显著相关(P<0.05);rs418440631G>A位点与乌珠穆沁羊4月龄胸围和管围以及6月龄胸围显著相关(P<0.05),与湖羊初生重和5月龄胸围极显著相关(P<0.01),与3月龄管围和5月龄体长和胸宽显著相关(P<0.05)。使用R语言corrplot包计算乌珠穆沁羊和湖羊体重与体尺的相关系数,结果表明湖羊和乌珠穆沁羊的体重与体尺呈现显著正相关(P<0.05),体重与胸围的相关系数最高。综上所述,PRSS12基因的rs402399470G>A和rs418440631G>A位点可作为绵羊生长性状的潜在分子遗传标记。本研究为肉羊育种和选育提供了参考依据。The identification of sheep(Ovis aries)molecular markers related to growth traits is of great significance to improve the economic benefits of sheep industry.Serine protease 12(PRSS12),also known as neurotrypsin,is specifically expressed in the central nervous system and is associated with a variety of neurological functions.In order to explore the polymorphism of PRSS12 gene and its correlation with the growth traits of Hu sheep and Ujumqin sheep,this study used gene chip technology to genotype 1008 Hu sheep and 336 Ujimqin sheep,and conducted association analysis with growth traits to identify molecular markers related to sheep growth and development.The results of genotyping showed that two SNP loci were detected in Ujimqin sheep population,in which rs402399470G>A presented low polymorphism,while rs418440631G>A showed moderate polymorphism.And only one SNP locus,namely rs418440631G>A,was detected in Hu sheep population,which showed low polymorphism.The results of association analysis with growth traits showed that rs402399470G>A locus was significantly correlated with 4-month-old body weight and 6-month-old chest width(P<0.05),while rs418440631G>A locus was significantly correlated with 4-month-old chest circumference,tube circumference and 6-month-old chest circumference of Ujimqin sheep(P<0.05).The rs418440631G>A locus was extremely significantly correlated with birth weight and 5-month chest circumference of Hu sheep(P<0.01),and significantly correlated with 3-month tube circumference,5-month body length and chest width of Hu sheep(P<0.05).The correlation coefficient between body weight and body size of Ujimqin sheep and Hu sheep was calculated by R language corrplot package,showing a significant positive correlation between weight and body size(P<0.05),with the highest correlation coefficient between weight and chest circumference.There was a significant positive correlation between body weight and body size,and the correlation coefficient between weight and chest circumference was the highest.In conclusio

关 键 词：丝氨酸蛋白酶12基因(PRSS12) 湖羊 乌珠穆沁羊 生长发育性状 关联分析 

分 类 号：S826[农业科学—畜牧学]