禽脑脊髓炎病毒VP1蛋白抗体间接ELISA检测方法的建立与临床应用  

Establishment and clinical application of an indirect ELISA method for detecting VP1 protein antibody of avian encephalomyelitis virus

作　　者：吕转平[1] 苏晓月[1] 唐思静[1] 王艳萍[1] 张多明 LYU Zhuanping;SU Xiaoyue;TANG Sijing;WANG Yanping;ZHANG Duoming(Xinjiang Bayingolin Vocational and Technical College,Korla,Xinjiang 841000,China;Xinjiang Qiangdu Animal Husbandry Technology Co.,Ltd.,Bayingolin,Xinjiang 841801,China)

机构地区：[1]新疆巴音郭楞职业技术学院 [2]新疆羌都畜牧科技有限公司

出　　处：《中国兽医学报》2024年第5期887-892,共6页Chinese Journal of Veterinary Science

基　　金：2022年新疆维吾尔自治区科技特派员基金资助项目(2022KF044)。

摘　　要：为建立1种禽脑脊髓炎病毒(avian encephalomyelitis virus, AEV)抗体检测方法,试验对AEV VP1蛋白进行截短表达,以表达蛋白为包被抗原建立检测AEV抗体的间接ELISA方法。结果显示,重组VP1蛋白以包涵体形式表达,大小为34 kDa;可与AEV阳性血清发生特异性反应;以VP1蛋白为包被抗原建立的间接ELISA方法检测ALV、IBDV、IBV、ILTV、NDV、AIV-H9阳性血清均为阴性;检测AEV抗体的最低检出效价为1∶51 200;与IDEXX试剂盒的符合率为95.24%;检测新疆地区1 127份AEV疫苗免疫鸡血清样品和879份未免疫鸡血清样品的免疫抗体阳性率和感染抗体阳性率分别为88.91%和8.19%。结果表明,建立的间接ELISA方法特异、敏感、准确,可用于临床中免疫抗体和野毒感染抗体的检测和筛查。To establish a method for detecting antibodies against avian encephalomyelitis virus(AEV),the truncated VP1 protein of AEV was expressed and used as the coating antigen to establish an indirect ELISA method for detecting AEV antibodies.The results showed that the recombinant VP1 protein was expressed in the form of an inclusion body with a size of 34 kDa and reacted specifically with AEV positive serum.The indirect ELISA method established was negative for detecting serum of ALV,IBDV,IBV,ILTV,NDV,AIV-H9.The lowest detectable titer for AEV antibodies was 1∶51200,with a coincidence rate of 95.24%with IDEXX reagent kit.The antibody positive rates were 88.91%and 8.19%for 1127 chicken serum samples immunized with AEV vaccine and 879 chicken serum samples with no vaccination,respectively in Xinjiang region.The indirect ELISA method established in this study was specific,sensitive,and accurate,and can be used for the detection and screening of AEV infection.

关 键 词：禽脑脊髓炎病毒 VP1蛋白 间接ELISA 临床应用 

分 类 号：S852.65[农业科学—基础兽医学]