cpe+产气荚膜梭菌cpe定位分析  

cpe localization of cpe~+Clostridium perfringens isolates

作　　者：姜艳芬[1] 钟建辉 张凯悦 尹婧夷 JIANG Yanfen;ZHONG Jianhui;ZHANG Kaiyue;YIN Jingyi(College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi 712100,China)

机构地区：[1]西北农林科技大学动物医学院,陕西杨凌712100

出　　处：《中国兽医学报》2024年第5期980-986,共7页Chinese Journal of Veterinary Science

基　　金：中央高校基本科研业务费专项资金资助项目(2452023072);陕西省重点研发计划资助项目(2021NY-026)。

摘　　要：产气荚膜梭菌(Clostridium perfringens)可引起人类食物中毒和许多非食源性人类胃肠道(GI)疾病。编码其肠毒素(Clostridium perfringens enterotoxin, CPE)的基因cpe若位于染色体的菌易引起食物中毒,cpe位于质粒的菌与非食源性GI疾病相关,因此本研究应用PCR检测、单位点序列分型分析(SLST)、耐热性测定,综合分析cpe+菌的cpe定位。结果显示,双重PCR检测29株cpe+菌仅扩增出600 bp的cpe目的条带,未检测出IS1470、IS1470-like、IS1151序列;通过sod、sodFPF PCR检测及SLST分析推测所有检测菌的cpe可能均位于染色体上;7-1和21-3的活菌及芽胞耐热性均高于42-2,推测两者cpe可能位于染色体,而42-2 cpe可能位于质粒上。结果为产气荚膜梭菌引起食物中毒和非食源性疾病的诊断和防控以及cpe的位置与所致疾病相关性的研究提供材料和科学依据。Clostridium perfringens(C.perfringens)can cause food poisoning in humans and many non-foodborne gastrointestinal(GI)diseases.C.perfringens enterotoxin(CPE),whose encoding gene cpe locates on the chromosome is prone to cause human food poisoning,while C.perfringens with cpe locates on the plasmid is associated with non-foodborne human GI diseases.In this study,the localization of cpe of cpe~(+)isolates were analyzed by PCR amplification,single-locus sequence typing(SLST)as well as the heat resistance of vegetative cells and spores.The duplex PCR results showed that only 600 bp bands were amplified from 29 cpe~+isolates,IS1470,IS1470-like and IS1151 target bands were not detected.The conventional sod and sodFPF PCR detection as well as SLST analysis showed that cpe of the detected isolates may locate on chromosome.The heat resistance of vegetative cells and spores of strain 7-1 and 21-3 were higher than that of 42-2,it was speculated that cpe of 7-1 and 21-3 locate on chromosomes while cpe of 42-2 locates on plasmid.In conclusion,it provides materials and scientific basis for the diagnosis,prevention and control of food poisoning and non-foodborne diseases,as well as the correlation between cpe location and the disease caused by C.perfringens.

关 键 词：产气荚膜梭菌 肠毒素基因 超氧化物歧化酶基因 单位点序列分型分析 芽胞 耐热性 

分 类 号：S852.61[农业科学—基础兽医学] R535[农业科学—兽医学]