β-谷甾醇对TGF-β1诱导的小鼠乳腺纤维化的影响  

Effect ofβ-sitosterol on TGF-β1-induced mammary fibrosis in mice

作　　者：徐平 阚兴池 黄雅萍 付守鹏 柳巨雄 XU Ping;KAN Xingchi;HUANG Yaping;FU Shoupeng;LIU Juxiong(College of Veterinary Medicine,Jilin University,Changchun 130062,China;Shanghai Animal Disease Prevention and Control Center,Shanghai 201103,China;Intelligent Com puting Platform Research Center of Zhejiang Laboratory,Hangzhou 311121,China)

机构地区：[1]吉林大学动物医学学院,吉林长春130062 [2]上海市动物疫病预防控制中心,上海201103 [3]之江实验室智能计算平台研究中心,浙江杭州311121

出　　处：《中国兽医学报》2024年第5期994-999,共6页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金青年基金资助项目(32302832);中国博士后研究基金资助项目(2023M733270)。

摘　　要：乳腺纤维化对奶牛养殖业造成巨大的经济损失,本研究的目的是探究β-谷甾醇(BSS)对TGF-β1诱导的小鼠乳腺纤维化是否具有缓解作用。试验分为空白对照组、模型组、模型+药物处理组,以乳腺灌注TGF-β1的方式构建小鼠乳腺纤维化模型,待模型构建完24 h收集小鼠乳腺组织。应用HE染色和Masson染色评价乳腺组织病理结构的变化情况;应用荧光定量PCR方法检测乳腺组织中collagenⅠ、α-SMA、vimentin (VIM)等纤维化相关蛋白的mRNA水平变化;应用Western blot和免疫荧光技术检测collagenⅡ、E-cadherin (E-cad)和α-SMA用于评价乳腺组织中纤维化蛋白表达情况。结果显示,BSS预处理后可以显著缓解TGF-β1诱导的小鼠乳腺病理损伤,显著抑制collagenⅠ、α-SMA、VIM mRNA水平的升高,显著抑制collagenⅡ、α-SMA蛋白水平的升高和E-cad蛋白水平的降低。BSS通过抑制纤维化表型指标的升高有效缓解纤维化病变的发生发展。This study aims to investigate the effect ofβ-sitosterol(BSS)on TGF-β1-induced mammary fibrosis in mice.The experiment was divided into the blank control group,model group,and model+drug group.Mouse mammary fibrosis model was constructed by infusing TGF-β1 into the mammary gland.After the model was constructed for 24 h,mouse mammary tissue was collected,and HE and Masson staining were used to evaluate the changes of mammary histopathology.mRNA levels of fibrosis-related collagenⅠ,α-SMA and VIM in breast tissues were detected by fluorescence quantitative PCR.Western blot detection of collagenⅡ,E-cad andα-SMA was used to evaluate fibrotic protein expression in mammary tissue.The results showed that in vivo experiments,BSS pretreatment could significantly alleviate the pathological damage of mammary gland induced by TGF-β1,and significantly inhibit the expression of collagenⅠ,α-SMA and VIM mRNA.The increase of collagenⅡ,α-SMA protein levels and the decrease of E-cad protein levels was significantly inhibited.

关 键 词：Β-谷甾醇 乳腺纤维化 细胞外基质 转化生长因子Β1 

分 类 号：S852.21[农业科学—基础兽医学]