原人参二醇对人卵巢癌细胞HO-8910增殖和凋亡的影响  

Effects of protopanaxadiol on the proliferation and apoptosis of human ovarian cancer cells HO-8910 through regulation

作　　者：聂瑞华 朱伟[2] 罗金艳[3] NIE Rui-hua;ZHU Wei;LUO Jin-yan(Qihuang Chinese Medicine Academy,Jiangxi University of Chinese Medicine,Nanchang 330025,Jiangxi Province,China;Departmentof Integrated Traditional Chinese Medicine&Western Medicine,Jiangxi ancer Hospital,Nanchang 330006,Jiangxi Province,China;Department of Ophthalmology and Otorhinolaryngology,Nanchang Hongdu Hospital of Traditional Chinese Medicine,Nanchang 330000,Jiangxi Province,China)

机构地区：[1]江西中医药大学岐黄国医书院,江西南昌330025 [2]江西省肿瘤医院中西医结合科,江西南昌330006 [3]南昌市洪都中医院五官科,江西南昌330000

出　　处：《中国临床药理学杂志》2024年第12期1769-1773,共5页The Chinese Journal of Clinical Pharmacology

基　　金：江西省中医药管理局科技计划基金资助项目(2020B0137)。

摘　　要：目的探讨原人参二醇对人卵巢癌细胞HO-8910增殖、凋亡的影响及作用机制。方法将人卵巢癌细胞HO-8910细胞随机分为空白组(0.9%NaCl)和低、中、高剂量实验组(分别用25、50、100 mg·kg^(-1)的原人参二醇处理细胞)。用细胞计数试剂盒-8(CCK-8)实验检测细胞增殖的抑制情况;用流式细胞术检测细胞凋亡情况;用细胞划痕实验检测细胞迁移情况;用Transwell实验检测细胞侵袭情况;用蛋白质印迹法检测磷脂酰肌醇-3激酶(PI3K)、磷酸化蛋白激酶B(p-AKT)、增殖细胞核抗原(PCNA)、B淋巴细胞瘤-2(Bcl-2)、Bcl-2相关X蛋白(Bax)的表达水平。结果空白组和低、中、高剂量实验组的增殖抑制率分别为(16.89±4.15)%、(28.43±3.66)%、(37.96±4.98)%和(50.11±5.24)%,凋亡率分别为(4.23±1.07)%、(12.36±2.79)%、(24.32±2.93)%和(42.40±3.28)%,PCNA蛋白相对表达水平分别为0.85±0.08、0.69±0.06、0.43±0.06和0.25±0.03,Bax蛋白相对表达水平分别为0.18±0.03、0.33±0.04、0.50±0.05和0.69±0.05,Bcl-2蛋白相对表达水平分别为0.82±0.07、0.63±0.04、0.47±0.05和0.30±0.03,迁移率分别为(52.33±4.25)%、(40.16±4.03)%、(29.63±3.25)%和(20.15±2.12)%,侵袭率分别为(60.26±5.88)%、(49.33±4.28)%、(30.15±3.68)%和(22.15±1.96)%,PI3K蛋白相对表达水平分别为0.48±0.04、0.34±0.04、0.26±0.03和0.15±0.01,p-AKT蛋白相对表达水平分别为0.45±0.03、0.35±0.02、0.23±0.03和0.13±0.02。低、中、高剂量实验组上述指标与空白组比较,差异均有统计学意义(均P<0.05)。结论原人参二醇可能通过阻断PI3K/AKT信号通路激活,调控PCNA、Bax、Bcl-2蛋白表达来抑制人卵巢癌细胞HO-8910增殖、迁移及侵袭并促进其凋亡,以此实现抗卵巢癌作用。Objective To investigate the effect of propanaxanediol on proliferation and apoptosis of human ovarian cancer cell HO-8910 and analyze the mechanism.Methods Human ovarian cancer cell HO-8910were randomly divided into blank group(0.9%NaCl)and experimental-L,-M,-H groups(25,50,100 mg·kg^(-1)propanaxanadiol treated cells,respectively).Cell counting kit-8(CCK-8)was used to detect the proliferation inhibition;the apoptosis was detected by flow cytometry;the migration was detected by cell scratch test;the invasion was detected by Transwell assay;the expression levels of phosphatidylinositol 3 kinase(PI3K),phosphorylated protein kinase B(p-AKT),proliferating cell nuclear antigen(PC NA),B cell lymphoma-2(Bel-2)and Bel-2 associated X protein(Bax)were detected by Western blot.Results The proliferation inhibition rates of blank group and experimental-L,-M,-H groups were(16.89±4.15)%,(28.43±3.66)%,(37.96±4.98)%and(50.11±5.24)%;the apoptosis rates were(4.23±1.07)%,(12.36±2.79)%,(24.32±2.93)%and(42.40±3.28)%;the expression levels of PCNA protein were 0.85±0.08,0.69±0.06,0.43±0.06 and 0.25±0.03;the expression levels of Bax protein were0.18±0.03,0.33±0.04,0.50±0.05 and 0.69±0.05;the expression levels of Bcl-2 protein were 0.82±0.07,0.63±0.04,0.47±0.05 and 0.30±0.03;the mobility were(52.33±4.25)%,(40.16±4.03)%,(29.63±3.25)%and(20.15±2.12)%;the invasion rates were(60.26±5.88)%,(49.33±4.28)%,(30.15±3.68)%and(22.15±1.96)%;the expression levels of PI3K protein were 0.48±0.04,0.34±0.04,0.26±0.03 and 0.15±0.01;the expression levels of p-AKT protein were 0.45±0.03,0.35±0.02,0.23±0.03and 0.13±0.02,respectively.There were statistically significant differences in the above indexes between the experimental-L,-M,-H groups and the blank group(all P<0.05).Conclusion Protopanaxadiol may inhibit the proliferation,migration and invasion of human ovarian cancer cell HO-8910 by blocking the activation of PI3K/AKT signaling pathway,regulating the expression of PCNA,Bax and Bel-2 proteins and promoting their apopt

关 键 词：原人参二醇 卵巢癌 蛋白激酶B B淋巴细胞瘤-2 增殖 凋亡 

分 类 号：R28[医药卫生—中药学]