白藜芦醇通过TGF-β1/Smad3信号转导途径改善间质性肺病模型大鼠的研究  

Resarch of resveratrol improving interstitial lung disease in rats through TGF-β1/Smad3 signaling transduction pathway

作　　者：祁晓静 张娜[1] 张月琴[1] QI Xiao-jing;ZHANG Na;ZHANG Yue-qin(Department of Respiratory Critical Care,Qinghai Provincial People's Hospital,Xining 810007,Qinghai Province,China)

机构地区：[1]青海省人民医院呼吸重症科,青海西宁810007

出　　处：《中国临床药理学杂志》2024年第12期1789-1793,共5页The Chinese Journal of Clinical Pharmacology

基　　金：青海省科技计划基金资助项目(2020-ZJ-930)。

摘　　要：目的研究白藜芦醇(RSV)改善间质性肺病(ILD)模型大鼠的作用机制。方法用博来霉素单次气管灌注给药构建ILD模型大鼠,随机分为模型组和低、中、高剂量实验组,每组12只;另取12只正常大鼠作为空白组。低、中、高剂量实验组分别灌胃给予25、50、100 mg·kg^(-1)RSV;空白组和模型组均灌胃给予等体积0.9%NaCl。5组大鼠每天给药1次,连续给药14 d。用酶联免疫吸附试验法检测肺组织中炎性因子和超氧化物歧化酶(SOD)水平,用蛋白质印迹法检测上皮间质转化(EMT)相关蛋白、转化生长因子-β1(TGF-β1)和Smad同源物3(Smad3)蛋白的表达水平。结果中、高剂量实验组和模型组、空白组的白细胞介素-1β水平分别为(7.32±0.33)、(5.07±0.39)、(11.28±0.72)和(3.78±0.12)pg·mL^(-1),SOD水平分别为(4.73±0.06)、(6.55±0.15)、(1.21±0.12)和(8.27±0.56)pg·mL^(-1),TGF-β1蛋白相对表达水平分别为0.49±0.06、0.34±0.04、0.78±0.08和0.11±0.02,Smad3蛋白相对表达水平分别为0.44±0.07、0.19±0.03、0.93±0.08和0.07±0.01;中、高剂量实验组的上述指标与模型组比较,在统计学上差异均有统计学意义(均P<0.05)。结论白藜芦醇通过抑制EMT和下调TGF-β1/Smad3信号通路的表达,从而发挥保护ILD大鼠的作用。Objective To investigate the mechanism of resveratrol(RSV)in interstitial lung disease(ILD).Methods The ILD model rats were established by administering a single intratracheal instillation of bleomycin.The ILD model rats were randomly divided into model group and experimental-L,-M,-H groups,with 12 rats each group,then chose another 12 normal rats as blank group.The experimental-L,-M,-H groups received 25,50 and 100 mg·kg^(-1)RSV by gavage;blank and model groups were given an equal volume of 0.9%NaCl by gavage.Five groups were treated for 14 days with once a day.The levels of pro-inflammatory factors,superoxide dismutase(SOD)in lung tissue were detected by enzyme linked immunosorbent assay.The expression levels of EMT-related proteins and transforming growth factor-β1(TGF-β1),SMAD family member 3(Smad3)were detected by Western blot.Results The levels of interleukin-1βin the experimental-M,-H groups,model group and blank group were(7.32±0.33),(5.07±0.39),(11.28±0.72)and(3.78±0.12)pg·mL^(-1);the levels of SOD were(4.73±0.06),(6.55±0.15),(1.21±0.12)and(8.27±0.56)pg·mL^(-1);the relative expression levels of TGF-β1 protein were 0.49±0.06,0.34±0.04,0.78±0.08 and 0.11±0.02;the relative expression levels of Smad3 protein were 0.44±0.07,0.19±0.03,0.93±0.08 and 0.07±0.01,respectively.The differences of above indexes were statistically significant between the model group and the experimental-M,-H groups(all P<0.05).Conclusion RSV can protect against ILD rats by inhibiting EMT and downregulating the TGF-β1/Smad3 signaling pathway.

关 键 词：白藜芦醇 间质性肺疾病 大鼠 博来霉素 转化生长因子-β1 

分 类 号：R979.5[医药卫生—药品]